This report covers the methodology for generation of stable heterohybridoma clones producing Foot-and-mouth disease virus (FMDV) reactive porcine monoclonal antibodies (mAbs). Swine received five inoculations of an inactivated O1 Manisa FMDV vaccine prior to the harvest of splenocytes. Due to the lack of a species-specific hybridoma fusion partner, the Sp2/0 murine myeloma cell line was utilized for the formation of porcine-murine heterohybridoma clones. Twenty-nine FMDV-reactive parental clones were generated. Following sub-cloning and monitoring of reactivity over 20 serial passages, eleven subclones derived from unique parental origins were characterized and are reported herein.

This methodology demonstrated the production of porcine mAbs by fusion of porcine splenocytes from immunized pigs with murine myeloma cells to generate heterohybridomas. The porcine immune response may differ from the murine immune response in relation to recognized epitopes. Therefore, application of this methodology may provide valuable resources for swine immunology and enhance the understanding of the mechanisms for antibody based protection from diseases in swine.

该报告涵盖了产生口蹄疫病毒（FMDV）反应性猪单克隆抗体（mAb）的稳定异源杂交瘤克隆的产生方法。在收获脾细胞之前，猪接受了五次灭活的O1 Manisa FMDV疫苗接种。由于缺乏物种特异性杂交瘤融合伴侣，Sp2 / 0鼠骨髓瘤细胞系被用于形成猪-鼠异种杂交瘤克隆。产生了29个FMDV反应性的亲本克隆。在进行亚克隆并监测了20个连续传代的反应性之后，表征了11个衍生自独特亲本来源的亚克隆，并在本文中进行了报道。

该方法论证明了通过将来自免疫猪的猪脾细胞与鼠骨髓瘤细胞融合以产生异种杂交瘤而产生猪单克隆抗体。就识别的表位而言，猪的免疫应答可能不同于鼠类的免疫应答。因此，该方法的应用可以为猪的免疫学提供有价值的资源，并增强对基于抗体的猪疾病防护机制的理解。