Integration of solid-liquid and liquid-liquid extractions, SLE and LLE, respectively, for selective recovery of Origanum vulgare subsp. glandulosum phenolics was studied. A Two-Phase Solvent Extraction (TPSE) was performed by maceration at room temperature using a green biphasic solvent system and compared to Centrifugal Partition Extraction (CPE). Up to 69 % of phenolics were similarly recovered by both CPE and TPSE techniques using the ethyl acetate/acetic acid/water solvent system (EAW) in immiscible proportions while only 56 % of purity was reached using the conventional successive technique. Up to 19 g GAE/100 g DW of total phenolics were recovered by solid-liquid-liquid extraction (SLLE) while only 12 g GAE/100 g DW was isolated by using SLE with EAW organic phase. Comparing TPSE to organic and aqueous macerations conducted separately, a clear positive impact of combination was observed, namely more than 57 % and 83 % enhancement of the extraction yields, respectively. Such extracts exhibited a very strong antioxidant activity as shown by the antioxidant activity index (AAI) higher than 2.

整合了固液萃取和液液萃取，分别用于SLE和LLE，以选择性地回收牛至子亚种。腺体研究了酚醛树脂。通过使用绿色双相溶剂系统在室温下浸渍进行两相溶剂萃取（TPSE），并将其与离心分配萃取（CPE）进行比较。通过CPE和TPSE技术，使用乙酸乙酯/乙酸/水溶剂系统（EAW）可以不混溶的比例回收多达69％的酚类，而使用常规连续技术只能达到56％的纯度。通过固-液-液萃取（SLLE）回收了多达19 g GAE / 100 g DW的总酚，而将SLE与EAW有机相分离仅分离了12 g GAE / 100 g DW。将TPSE与分别进行的有机浸渍法和水性浸渍法进行比较，观察到了组合的明显积极影响，即萃取率分别提高了57％和83％以上。