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Global profiling of lncRNAs-miRNAs-mRNAs reveals differential expression of coding genes and non-coding RNAs in the lung of beagle dogs at different stages of Toxocara canis infection
International Journal for Parasitology ( IF 4 ) Pub Date : 2020-09-28 , DOI: 10.1016/j.ijpara.2020.07.014
Wen-Bin Zheng 1 , Yang Zou 2 , Jun-Jun He 2 , Hany M Elsheikha 3 , Guo-Hua Liu 4 , Min-Hua Hu 5 , Shui-Lian Wang 4 , Xing-Quan Zhu 6
Affiliation  

The roundworm Toxocara canis causes toxocariasis in dogs and larval migrans in humans. Better understanding of the lung response to T. canis infection could explain why T. canis must migrate to and undergoes part of its development inside the lung of the definitive host. In this study, we profiled the expression patterns of long non-coding RNAs (lncRNAs), microRNAs (miRNAs), and mRNAs in the lungs of Beagle dogs infected by T. canis, using high throughput RNA sequencing. At 24 h p.i., 1,012 lncRNAs, 393 mRNAs and 10 miRNAs were differentially expressed (DE). We also identified 883 DElncRNAs, 264 DEmRNAs and 20 DEmiRNAs at 96 h p.i., and 996 DElncRNAs, 342 DEmRNAs and eight DEmiRNAs at 36 days p.i., between infected and control dogs. Significant changes in the levels of expression of transcripts related to immune response and inflammation were associated with the antiparasitic response of the lung to T. canis. The remarkable increase in the expression of scgb1a1 at all time points after infection suggests the need for consistent moderation of the excessive inflammatory response. Also, upregulation of foxj1 at 24 h p.i., and downregulation of IL-1β and IL-21 at 96 h p.i., suggest an attenuation of the humoral immunity of infected dogs. These results indicate that T. canis pathogenesis in the lung is mediated through contributions from both pro-inflammatory and anti-inflammatory mechanisms. Competing endogenous RNA (ceRNA) network analysis revealed significant interactions between DElncRNAs, DEmiRNAs and DEmRNAs, and improved our understanding of the ceRNA regulatory mechanisms in the context of T. canis infection. These data provide comprehensive understanding of the regulatory networks that govern the lung response to T. canis infection and reveal new mechanistic insights into the interaction between the host and parasite during the course of T. canis infection in the canine.



中文翻译:

lncRNAs-miRNAs-mRNAs的全局分析揭示了犬弓首蛔虫感染不同阶段比格犬肺中编码基因和非编码RNA的差异表达

蛔虫犬弓蛔虫引起狗和幼虫移行人类弓蛔虫病。更好地理解肺对T. canis感染的反应可以解释为什么T. canis必须迁移到最终宿主的肺内并在其中进行部分发育。在这项研究中,我们分析了长链非编码 RNA (lncRNAs)、microRNAs (miRNAs) 和 mRNAs 在被犬T. canis感染的比格犬肺部的表达模式使用高通量 RNA 测序。在感染后 24 小时,1,012 个 lncRNA、393 个 mRNA 和 10 个 miRNA 差异表达(DE)。我们还在感染后 96 小时鉴定了 883 个 DElncRNA、264 个 DEmRNA 和 20 个 DEmiRNA,并在感染后 36 天鉴定了 996 个 DElncRNA、342 个 DEmRNA 和 8 个 DEmiRNA。与免疫反应和炎症相关的转录物表达水平的显着变化与肺对犬毛丝虫的抗寄生虫反应有关。感染后所有时间点scgb1a1表达的显着增加表明需要持续调节过度的炎症反应。此外,感染后 24 小时Foxj1上调,IL-1β感染后 96 小时的IL-21表明受感染狗的体液免疫减弱。这些结果表明肺中犬毛丝虫的发病机制是通过促炎和抗炎机制的贡献介导的。竞争性内源 RNA (ceRNA) 网络分析揭示了 DElncRNA、DEmiRNA 和 DEmRNA 之间的显着相互作用,并提高了我们对毛丝虫感染背景下 ceRNA 调控机制的理解。这些数据提供了对控制犬毛丝虫感染肺部反应的调控网络的全面了解,并揭示了犬毛丝虫病程中宿主和寄生虫之间相互作用的新机制见解 犬的感染。

更新日期:2020-09-28
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