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Morphological and physiological responses of tara ( Caesalpinia spinosa (Mol.) O. Kuntz) microshoots to ventilation and sucrose treatments
In Vitro Cellular & Developmental Biology - Plant ( IF 2.6 ) Pub Date : 2020-09-28 , DOI: 10.1007/s11627-020-10104-w
Jenny Elizabeth Núñez-Ramos , Elisa Quiala , Laisyn Posada , Saúl Mestanza , Leidy Sarmiento , Dion Daniels , Carlos R. Arroyo , Blanca Naranjo , Karla Vizuete , Carlos Noceda , Rafael Gómez-Kosky

One of the main problems of in vitro plant tissue culture is the poor ventilation in the culture vessels. This leads to morphological and physiological anomalies in regenerated in vitro plants, a crucial issue in woody species, such as Caesalpinia spinosa (Mol.) O. Kuntz, which is a legominous tree or thorny shrub of the Andes, adequate photoautotrophic and photomixotrophic in vitro rooting systems could eliminate these problems. Therefore, the purpose of the present study was to evaluate photoautotrophic and photomixotrophic treatments to optimize the in vitro rooting of C. spinosa microshoots. Micropropagated shoots were cultured in vessels sealed with a polypropylene lid with 0, 1, or 3 ventilation holes covered with a 0.45-μm adhesive polypropylene microfilter. The vessels contained MS salts with or without 30 g L−1 sucrose or, alternatively, a porous substrate (PRO-MIX®), which facilitated rooting and produced greater fresh and dry weights, a higher concentration of photosynthetic pigments and phenolic compounds, and more developed leaves. The optimal performance and higher survival was obtained by using PRO-MIX® with three filters for increased ventilation. For treatments in agar, only the one with sucrose and highly ventilated (three filters) reached a performance approaching that with PRO-MIX®. To date, the presented results have not been reported in scientific literature for C. spinosa.



中文翻译:

塔拉(Caesalpinia spinosa(Mol。)O.Kuntz)微芽对通风和蔗糖处理的形态和生理响应

体外植物组织培养的主要问题之一是培养容器中的通风不良。这导致形态和生理异常的再生体外厂,木本植物,如一个关键问题塔拉(摩尔)O.孔茨,这是一个legominous树或安第斯山脉的棫,充足的光合自养和光混合体外生根系统可以消除这些问题。因此,本研究的目的是评估光自养和光混合处理,以优化体外生根C.刺微拍。将微繁殖的枝条培养在用聚丙烯盖密封的容器中,该聚丙烯盖带有覆盖有0.45-μm粘性聚丙烯微滤器的0、1或3个通风孔。容器中含有MS盐(含或不含30 g L -1蔗糖)或多孔底物(PRO-MIX®),可促进生根并产生更大的鲜重和干重,更高浓度的光合色素和酚类化合物,以及叶片较发达。通过使用带有三个过滤器的PRO-MIX®可以提高通风性能,从而获得最佳性能和更高的存活率。对于琼脂处理,只有蔗糖和高度通风的过滤器(三个过滤器)才能达到接近PRO-MIX®的性能。迄今为止,目前的结果尚未在科学文献中报道。C. spinosa

更新日期:2020-09-28
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