The study employed in vitro assay to examine the peculiarities of immune status in children with functional disorders of the autonomic nervous system diagnosticated as asthenoneurotic syndrome. In contrast to control children without asthenoneurotic syndrome, the examined group was characterized by significantly (p<0.05) elevated hapten-specific immunological sensitization (indicated by anti-Al IgG), induction of inflammatory reactions (IL-1), activation of apoptosis (CD3+CD95+ and р53) observed against the background inhibition of adaptive immune response (CD3+, CD3+CD4+, CD3+CD8+, CD16+56+, and CD19+), as well as hyperexpression of glutamic acid, NO, and VEGF combined with deficiency of serotonin. In cultured immunocompetent cells derived from children with hapten-modified immune status, the combined application of cytokine stimulator IL-1 with hapten sensitizer aluminum or with endocrine stimulator cortisol significantly (p<0.05) up-regulated expression of IL-8 and IL-10, but down-regulated production of IL-17 in a dose-dependent manner.

中文翻译：

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半抗原诱导的软神经病综合征儿童免疫调节的体外评估

该研究采用体外试验来检查诊断为虚弱神经症综合征的自主神经系统功能障碍儿童的免疫状态的特殊性。与没有软神经病综合征的对照儿童相比，受检组的特征是显着（p<0.05）升高的半抗原特异性免疫致敏（由抗 Al IgG 表示）、炎症反应的诱导（IL-1）、细胞凋亡的激活（ CD3+CD95+ 和 р53) 观察到背景抑制适应性免疫反应（CD3+、CD3+CD4+、CD3+CD8+、CD16+56+ 和 CD19+），以及谷氨酸、NO 和 VEGF 的过度表达与缺陷相结合血清素。在来自具有半抗原修饰免疫状态的儿童的培养免疫活性细胞中，