Most of the lignocellulosic biomass residues are decomposed and transformed by a variety of microbes in the natural environment. The xylanase production from Aspergillus niger strain BG has been produced using wheat bran under solid-state fermentation (SSF). One factor at a time approach (OFAT) was used to optimize the effect of the incubation period, initial pH, moisture content, and cultivation temperature on the xylanase production. Furthermore, experiments were designed with a Box–Behnken design (BBD) on the same variables using response surface methodology (RSM). Analysis of variance (ANOVA) was carried out and the xylanase production was expressed with a mathematical equation depending on the factors. Maximum xylanase yield after OFAT approach and RSM optimization was significant with maximum values of 4008.25 ± 3.73 U/g of dry substrate (U/gds) and 5427.51 ± 4.4 U/gds which have been recorded respectively compared with the initial conditions (1899.02 ± 1.6 U/gds) after 7 days of fermentation. The effects of individual, interaction, and square terms on xylanase production were represented using the non-linear regression equations with significant R² and p values. The optimum conditions established by RSM method for the maximum xylanase production were obtained with a pH media of 2.5 at 37 °C using wheat bran as 84% humidified substrate after 66 h of incubation, this conditions resulted in 65.01% increased level of the xylanase production than produced in the initial conditions. Xylanase production from Aspergillus niger strain BG using RSM is considered advantageous for bioconversion of the agriculture residues.

在自然环境中，大多数木质纤维素生物质残留物会被各种微生物分解和转化。黑曲霉的木聚糖酶生产BG菌株已在固态发酵（SSF）下使用麦麸生产。使用一个时间因素（OFAT）来优化孵育时间，初始pH，水分含量和培养温度对木聚糖酶生产的影响。此外，使用响应表面方法（RSM）对相同变量进行Box-Behnken设计（BBD）设计实验。进行方差分析（ANOVA），并根据因素用数学方程式表示木聚糖酶的产生。OFAT方法和RSM优化后的最大木聚糖酶产量非常显着，分别记录了与初始条件相比的干底物（U / gds）的最大值4008.25±3.73 U / g和5427.51±4.4 U / gds的初始值（1899.02±1.6） U / gds）发酵7天后。R ²和p值。通过RSM方法确定的最大木聚糖酶生产的最佳条件是在37°C的pH值为2.5的条件下孵育66 h后使用麦麸作为84％加湿的底物获得的，该条件导致木聚糖酶生产水平提高了65.01％比初始条件下生产的要多。使用RSM从黑曲霉菌株BG生产木聚糖酶被认为有利于农业残留物的生物转化。