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Inhibition of chikusetsusaponin IVa on inflammatory responses in RAW264.7 cell line via MAPK pathway
Zeitschrift für Naturforschung C ( IF 2 ) Pub Date : 2020-09-28 , DOI: 10.1515/znc-2019-0107
Guangren Xu 1 , Hongyu Lei 2 , Qiaoling Yuan 1 , Huiyu Chen 2 , Jianming Su 1
Affiliation  

Chikusetsusaponin IVa (CHS-IVa), a saponin from herb Panacis japonicas, possesses extensive biological activities. However, the roles and underlying mechanisms of CHS-IVa on inflammation have not been fully clarified in the setting of murine macrophages. In this study, we found that CHS-IVa effectively reduced the expression of inflammatory mediators, including interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10), interleukin-1β (IL-1β), cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated murine macrophage-like RAW264.7 cells. Meanwhile, CHS-IVa could also evidently bate the contents of nitric oxide (NO) and prostaglandin E2 (PGE2) in cell culture supernatants. Furthermore, the anti-inflammatory activity of CHS-IVa may be via diminishing the phosphorylation of extracellular regulated protein kinases (ERK), p38, and c-Jun N-terminal kinase (JNK). Collectively, these findings will help to understand of the anti-inflammatory effects and mechanisms of P. japonicas deeply, and suggest a validated therapeutic use as an anti-inflammatory medication.

中文翻译:

竹节皂苷IVa通过MAPK通路抑制RAW264.7细胞系炎症反应

Chikusetsusaponin IVa (CHS-IVa) 是一种来自药材三七的皂苷,具有广泛的生物活性。然而,在小鼠巨噬细胞的环境中,CHS-IVa 对炎症的作用和潜在机制尚未完全阐明。在本研究中,我们发现 CHS-IVa 有效降低炎症介质的表达,包括白介素 6 (IL-6)、肿瘤坏死因子-α (TNF-α)、白介素 10 (IL-10)、白介素-脂多糖 (LPS) 刺激的小鼠巨噬细胞样 RAW264.7 细胞中的 1β (IL-1β)、环氧合酶 (COX-2)、诱导型一氧化氮合酶 (iNOS)。同时,CHS-IVa 还可以明显降低细胞培养上清液中一氧化氮 (NO) 和前列腺素 E2 (PGE2) 的含量。此外,CHS-IVa 的抗炎活性可能是通过减少细胞外调节蛋白激酶 (ERK)、p38 和 c-Jun N 端激酶 (JNK) 的磷酸化来实现的。总的来说,这些发现将有助于深入了解 P. japonicas 的抗炎作用和机制,并提出一种经过验证的抗炎药物治疗用途。
更新日期:2020-09-28
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