Metabolic syndrome and obesity have become serious threats to public health worldwide. This study was conducted to evaluate the anti-adipogenesis and metabolism-regulating effects of heat-inactivated Streptococcus thermophilus MN-ZLW-002 (MN-ZLW-002), which can be used as a yogurt starter. In vitro study suggested that MN-ZLW-002 stimulated the RAW264.7 macrophages to produce significant amounts of interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α and induced intense phosphorylation of P38, p44/42 MAPK, and nuclear factor κB. MN-ZLW-002-stimulated RAW264.7-conditioned medium (CM) notably suppressed the differentiation and adipogenesis of 3T3-L1 pre-adipocytes. The 12-week in vivo study suggested that orally administered MN-ZLW-002 significantly reduced the weight gain of mice caused by the high-fat diet (HFD) at weeks 3-8; decreased fasting blood glucose levels at week 4 and week 8; decreased serum total triglyceride level at week 12. MN-ZLW-002 also reduced serum IL-1β and chemokine ligand 3 levels in the HFD-fed mice. These findings suggest that heat-inactivated MN-ZLW-002 can suppress adipocytes differentiation and lipid accumulation by regulating the immune response, possibly via the release of cytokines, particularly TNF-α; MN-ZLW-002 can improve metabolism-related indicators in the early stage of HFD intervention and regulate the related pro-inflammatory immune response.

中文翻译：

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热灭活嗜热链球菌MN-ZLW-002的抗脂肪生成和代谢调节作用

代谢综合征和肥胖已成为全球公共卫生的严重威胁。本研究旨在评估可用作酸奶发酵剂的热灭活嗜热链球菌 MN-ZLW-002 (MN-ZLW-002) 的抗脂肪生成和代谢调节作用。体外研究表明，MN-ZLW-002 刺激 RAW264.7 巨噬细胞产生大量的白细胞介素 (IL)-6、IL-10 和肿瘤坏死因子 (TNF)-α，并诱导 P38、p44/ 42 MAPK 和核因子 κB。MN-ZLW-002 刺激的 RAW264.7 条件培养基 (CM) 显着抑制了 3T3-L1 前脂肪细胞的分化和脂肪生成。12周的体内研究表明，在第3-8周，口服MN-ZLW-002显着降低了由高脂饮食（HFD）引起的小鼠体重增加；在第 4 周和第 8 周降低空腹血糖水平；在第 12 周降低血清总甘油三酯水平。MN-ZLW-002 还降低了 HFD 喂养小鼠的血清 IL-1β 和趋化因子配体 3 水平。这些发现表明，热灭活的 MN-ZLW-002 可以通过调节免疫反应来抑制脂肪细胞分化和脂质积累，可能是通过释放细胞因子，特别是 TNF-α；MN-ZLW-002可在HFD干预早期提高代谢相关指标，调节相关促炎免疫反应。这些发现表明，热灭活的 MN-ZLW-002 可以通过调节免疫反应来抑制脂肪细胞分化和脂质积累，可能是通过释放细胞因子，特别是 TNF-α；MN-ZLW-002可在HFD干预早期提高代谢相关指标，调节相关促炎免疫反应。这些发现表明，热灭活的 MN-ZLW-002 可以通过调节免疫反应来抑制脂肪细胞分化和脂质积累，可能是通过释放细胞因子，特别是 TNF-α；MN-ZLW-002可以提高HFD干预早期代谢相关指标，调节相关促炎免疫反应。