Photosynthesis is especially sensitive to environmental conditions, and the composition of the photosynthetic apparatus can be modulated in response to environmental change, a process termed photosynthetic acclimation. Previously, we identified a role for a cytosolic fumarase, FUM2 in acclimation to low temperature in Arabidopsis thaliana. Mutant lines lacking FUM2 were unable to acclimate their photosynthetic apparatus to cold. Here, using gas exchange measurements and metabolite assays of acclimating and non‐acclimating plants, we show that acclimation to low temperature results in a change in the distribution of photosynthetically fixed carbon to different storage pools during the day. Proteomic analysis of wild‐type Col‐0 Arabidopsis and of a fum2 mutant, which was unable to acclimate to cold, indicates that extensive changes occurring in response to cold are affected in the mutant. Metabolic and proteomic data were used to parameterize metabolic models. Using an approach called flux sampling, we show how the relative export of triose phosphate and 3‐phosphoglycerate provides a signal of the chloroplast redox state that could underlie photosynthetic acclimation to cold.

中文翻译：

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来自拟南芥叶绿体的代谢通量提供控制光合适应寒冷的信号

光合作用对环境条件特别敏感，可以响应环境变化来调节光合作用设备的组成，这一过程称为光合适应。以前，我们确定了拟南芥中低温的胞质富马酸酶FUM2的作用。缺少FUM2的突变株无法使其光合设备适应寒冷。在这里，通过对适应环境和非适应环境的植物进行气体交换测量和代谢产物分析，我们发现适应低温会导致白天光合固定碳在不同存储池中的分布发生变化。野生型Col-0拟南芥和fum2的蛋白质组学分析不能适应寒冷的突变体表明，响应于寒冷而发生的广泛变化会影响突变体。代谢和蛋白质组学数据用于参数化代谢模型。使用一种称为通量采样的方法，我们展示了磷酸三糖和3-磷酸甘油酸的相对输出如何提供叶绿体氧化还原状态的信号，该信号可能是光合作用适应寒冷的基础。