Droplet microfluidics is a powerful platform for high-throughput single-molecule protein analysis. However, the issues of coalescence and crosstalk of droplets compromise the accuracy of detection and hinder its wide application. To address these limitations, a novel colloidosome-based method was presented by combining a Pickering emulsion with droplet microfluidics for single-molecule protein analysis. Utilizing the self-assembly of easily synthesized colloidal surfactant F-SiO₂ NPs at the water/oil interface, the colloidosomes are rigidly stabilized and can effectively avoid the leakage of fluorescent molecules. The crosstalk-free colloidosomes enable high-throughput single-molecule protein analysis, including heterogenous dynamic studies and digital detection. As a robust and accurate method, colloidosome-based microfluidics is promising as a powerful tool for a wide variety of applications, such as directed enzyme evolution, digital enzyme-linked immunosorbent assay (ELISA), and screening of antibiotics.

液滴微流控技术是用于高通量单分子蛋白质分析的强大平台。然而，液滴的聚结和串扰的问题损害了检测的准确性，并阻碍了其广泛的应用。为了解决这些局限性，提出了一种新颖的基于胶体的方法，该方法通过将Pickering乳液与液滴微流控技术相结合来进行单分子蛋白质分析。利用易于合成的胶体表面活性剂F-SiO ₂的自组装在水/油界面处的NP，胶体是刚性稳定的，可以有效避免荧光分子的泄漏。无串扰的胶体使高通量的单分子蛋白质分析成为可能，包括异源动力学研究和数字检测。作为一种可靠且准确的方法，基于胶体的微流体有望作为强大的工具用于各种应用，例如定向酶进化，数字酶联免疫吸附测定（ELISA）和抗生素筛选。