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Beauvericin alters the expression of genes coding for key proteins of the mitochondrial chain in ovine cumulus-oocyte complexes.
Mycotoxin Research ( IF 3 ) Pub Date : 2020-09-26 , DOI: 10.1007/s12550-020-00409-5
Antonella Mastrorocco 1, 2 , Elena Ciani 1 , Luigi Nicassio 1 , Bernard A J Roelen 3 , Fiorenza Minervini 4 , Maria Elena Dell'Aquila 1
Affiliation  

Beauvericin (BEA) is a member of the enniatin family of mycotoxins which has received increasing interest because of frequent occurrence in food and feed. By its ionophoric properties, BEA is able to alter membrane ion permeability uncoupling oxidative phosphorylation. It was also shown to alter oocyte mitochondrial function. In this study, the effects of BEA at 0.5, 1, ,3 and 5 μmol/L on expression of genes coding for key proteins of the mitochondrial chain in ovine oocytes and cumulus cells were evaluated at different time points of in vitro maturation (IVM), germinal vesicle (GV; t = 0), metaphase I (MI; t = 7 h), and metaphase II (MII; t = 24 h). The expression of nuclear (TFAM, NDUFA12, UQCRH, COX4, ATP5O) and mitochondrial (ND1, COX1, COX2, ATP6, ATP8) genes coding for proteins of Complexes I, III, IV, and V was analyzed by qRT-PCR. After BEA exposure, perturbed expression of all genes was observed in cumulus cells and in oocytes at the MI stage (7 h IVM). Expression of ND1, UQCRH, COX4 and ATP5O was downregulated in cumulus cells and upregulated in oocytes starting from 0.5 μmol/L BEA. Expression of TFAM, NDUFA12, COX1, COX2, ATP6, and ATP8 was upregulated starting from 1 μmol/L in cumulus cells and from 3 μmol/L in oocytes. Cumulus cells and oocytes displayed different gene expression patterns upon BEA exposure. The downregulation in cumulus cells of four genes coding for proteins of mitochondrial complexes could represent a major toxic event induced by BEA on the cumulus-oocyte complex which may result in mitochondrial functional alteration.



中文翻译:

Beauvericin 改变编码绵羊卵丘-卵母细胞复合物中线粒体链关键蛋白的基因的表达。

白僵菌素 (BEA) 是霉菌毒素的 enniatin 家族的一员,由于在食品和饲料中频繁出现,它越来越受到关注。通过其离子载体特性,BEA 能够改变膜离子通透性,解偶联氧化磷酸化。它还被证明可以改变卵母细胞线粒体功能。本研究评估了 0.5、1、3 和 5 μmol/L 的 BEA 在体外成熟 (IVM) 的不同时间点对绵羊卵母细胞和卵丘细胞线粒体链关键蛋白编码基因表达的影响。 )、生发囊泡 (GV; t = 0)、中期 I (MI; t = 7 h) 和中期 II (MII; t = 24 h)。核表达(TFAM , NDUFA12 ,通过 qRT-PCR 分析了编码复合物 I、III、IV 和 V 蛋白质的UQCRHCOX4ATP5O)和线粒体(ND1COX1COX2ATP6ATP8)基因。BEA 暴露后,在卵丘细胞和 MI 阶段(7 小时 IVM)的卵母细胞中观察到所有基因的扰动表达。从 0.5 μmol/L BEA 开始,ND1UQCRHCOX4ATP5O 的表达在卵丘细胞中下调,而在卵母细胞中上调。表达TFAMNDUFA12COX1COX2ATP6ATP8从卵丘细胞中的 1 μmol/L 和卵母细胞中的 3 μmol/L 开始上调。BEA 暴露后,卵丘细胞和卵母细胞显示出不同的基因表达模式。卵丘细胞中四种编码线粒体复合物蛋白质的基因下调可能代表 BEA 对卵丘 - 卵母细胞复合物诱导的主要毒性事件,这可能导致线粒体功能改变。

更新日期:2020-09-28
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