Hydrogen peroxide (H₂O₂) is an important signaling molecule and plays key roles in multiple plant physiological processes. The rapid and direct monitoring of H₂O₂ could improve our understanding of its regulatory mechanisms in plants. In this study, we developed a paper-based analytical device consisting of a disposable nano-gold modified indium tin oxide working electrode to provide a platform for the rapid and direct detection of H₂O₂. The total analytical time was dramatically shortened to be approximate 3 min due to the avoidance of the time-consuming and complex treatment of plant samples. In addition, the amount of plant samples required was less than 3 mg in our approach. We used this system to monitor the concentrations of H₂O₂ in tomato leaves infected by Botrytiscinerea within 24 h. Our results showed that the concentration of H₂O₂ in tomato leaves was increased in the initial phase, peaked at 1.5 μmol gFW⁻¹ at 6 h, and then decreased. The production trend of H₂O₂ in tomato leaves inoculated with Botrytiscinerea detected with our approach is similar to the 3,3-diaminobenzidine staining method. Taken together, our study offers a rapid and direct approach for the detection of H₂O₂, which will not only pave the way for the further investigation of the regulation mechanisms of H₂O₂ in plants, but also promote the development of precision agriculture technology.

中文翻译：

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基于aper的分析装置，用于灰葡萄孢菌接种的番茄叶片中快速，直接电化学检测过氧化氢

过氧化氢（H ₂ O ₂）是重要的信号分子，在多种植物生理过程中起着关键作用。H ₂ O ₂的快速直接监测可以增进我们对植物中其调节机制的了解。在这项研究中，我们开发了一种基于纸的分析装置，该装置由一次性纳米金改性的铟锡氧化物工作电极组成，为快速，直接检测H ₂ O ₂提供了平台。。由于避免了耗时且复杂的植物样品处理，总的分析时间显着缩短为大约3分钟。此外，在我们的方法中，所需的植物样品量少于3 mg。我们使用该系统监测在24小时内被灰葡萄孢菌感染的番茄叶片中H ₂ O ₂的浓度。我们的结果表明，番茄叶片中的H ₂ O ₂浓度在初始阶段增加，在6 h达到1.5μmolgFW ^-1，然后下降。灰葡萄孢菌接种番茄叶片中H ₂ O ₂的产生趋势用我们的方法检测到的结果与3,3-二氨基联苯胺染色方法相似。综上所述，我们的研究提供了一种快速，直接的H ₂ O ₂检测方法，不仅为进一步研究植物中H ₂ O ₂的调控机制铺平了道路，而且还促进了精密度的发展。农业技术。