Cell sheet morphogenesis is essential for metazoan development and homeostasis of animal form – it contributes to developmental milestones including gastrulation, neural tube closure, heart and palate formation and to tissue maintenance during wound healing. Dorsal closure, a well-characterized stage in Drosophila embryogenesis and a model for cell sheet morphogenesis, is a remarkably robust process during which coordination of conserved gene expression patterns and signaling cascades regulate the cellular shape changes and movements. New ‘dorsal closure genes’ continue to be discovered due to advances in imaging and genetics. Here, we extend our previous study of the right arm of the 2^nd chromosome to the left arm of the 2^nd chromosome using the Bloomington deficiency kit’s set of large deletions, which collectively remove 98.9% of the genes on the left arm of chromosome two (2L) to identify ‘dorsal closure deficiencies’. We successfully screened 87.2% of the genes and identified diverse dorsal closure defects in embryos homozygous for 49 deficiencies, 27 of which delete no known dorsal closure gene. These homozygous deficiencies cause defects in cell shape, canthus formation and tissue dynamics. Within these deficiencies, we have identified pimples, odd-skipped, paired, and sloppy-paired 1 as dorsal closure genes on 2L that affect lateral epidermal cells. We will continue to identify novel ‘dorsal closure genes’ with further analysis. These forward genetic screens are expected to identify new processes and pathways that contribute to closure and links between pathways and structures already known to coordinate various aspects of closure.

细胞表形态的形成对于后生动物的发展和动物形态的稳态至关重要-它有助于发育里程碑，包括胃造瘘，神经管闭合，心脏和pa的形成以及伤口愈合过程中的组织维持。背闭合是果蝇胚胎发生中一个充分表征的阶段，是细胞表形态发生的模型，是一个非常健壮的过程，在此过程中，保守基因表达模式和信号级联的协调调节了细胞的形状变化和运动。由于成像和遗传学的进步，新的“背闭合基因”继续被发现。在这里，我们我们以前的2右臂的研究范围扩大^第二染色体2的左臂^次染色体使用Bloomington缺陷试剂盒的大缺失集，可共同删除2号染色体（2L）左臂上98.9％的基因，以鉴定“背闭合缺陷”。我们成功地筛选了87.2％的基因，并在纯合子中鉴定了49个缺陷的纯合子中不同的背闭合缺陷，其中27个缺失了已知的背闭合基因。这些纯合缺陷导致细胞形状，can形成和组织动力学方面的缺陷。在这些不足中，我们发现了丘疹，跳过， 配对的和 马虎配对1作为影响2L外侧表皮细胞的背闭合基因。我们将继续进行进一步分析，以确定新的“背闭合基因”。这些前瞻性遗传筛选有望识别出有助于闭合的新过程和途径，以及已知在协调闭合各方面的途径与结构之间的联系。