Background

Lactobacillus reuteri I5007 possesses many excellent probiotic characteristics in piglets. miRNA plays important role in host-microbiota interactions, but the mechanism by which L. reuteri I5007 regulates intestinal function through its influence on miRNA expression is unknown.

Objective

This study analyzed the miRNA expression patterns in the ileum mucosa tissue of piglets by L. reuteri I5007 treatment, aim to clarify its molecular mechanism for regulating intestinal function through miRNA.

Methods

Neonatal piglets were orally administered L. reuteri I5007 or a placebo daily starting on day 1, and differential expression of ileal miRNAs was analyzed at 10 and 20 days of age by small RNA sequencing.

Results

361 known porcine miRNAs were identified, and ten miRNAs were highly expressed in the ileum mucosa in both treatments. Nineteen differentially expressed (DE) miRNAs were identified in response to L. reuteri treatment, and four DE miRNAs (ssc-miR-196a, -196b-5p, -1285 and -10386) were differentially expressed at both time points. The KEGG pathway analyses showed the targets of 19 DE miRNAs were involved in 63 significantly enriched pathways, including the PI3K-Akt and MAPK pathways, which were confirmed to play important roles in probiotic-host communication. L. reuteri I5007 exerted anti-inflammatory effects by influencing the levels of inflammatory cytokines. Suppressor of cytokine signalling 4 gene was the target gene of ssc-miR-196a/-196b-5p, overexpression of ssc-miR-196a/-196b-5p downregulated the mRNA expression of IL-1β and TNFα in IPEC-J2 cells.

Conclusion

Our study provides new insight into the role of miRNAs in the intestinal function of piglets after L. reuteri I5007 treatment.

中文翻译：

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罗伊氏乳杆菌处理仔猪回肠黏膜后差异表达miRNA的鉴定

背景

罗伊氏乳杆菌I5007 在仔猪体内具有许多优良的益生菌特性。miRNA 在宿主-微生物群相互作用中发挥重要作用，但罗伊氏乳杆菌I5007 通过其对 miRNA 表达的影响来调节肠道功能的机制尚不清楚。

客观的

本研究分析了罗伊氏乳杆菌I5007处理仔猪回肠黏膜组织中miRNA的表达模式，旨在阐明其通过miRNA调节肠道功能的分子机制。

方法

新生仔猪从第 1 天开始每天口服罗伊氏乳杆菌I5007 或安慰剂，并通过小 RNA 测序分析在 10 和 20 日龄时回肠 miRNA 的差异表达。

结果

鉴定了 361 种已知的猪 miRNA，两种处理中的回肠黏膜中有 10 种 miRNA 高表达。响应罗伊氏乳杆菌处理鉴定了 19 种差异表达 (DE) miRNA ，4 种 DE miRNA（ssc-miR-196a、-196b-5p、-1285 和 -10386）在两个时间点均差异表达。KEGG 通路分析表明，19 个 DE miRNA 的靶标参与了 63 个显着富集的通路，包括 PI3K-Akt 和 MAPK 通路，这些通路被证实在益生菌-宿主通讯中发挥重要作用。罗伊氏乳杆菌I5007 通过影响炎性细胞因子的水平发挥抗炎作用。细胞因子信号抑制因子4基因是ssc-miR-196a/-196b-5p的靶基因，ssc-miR-196a/-196b-5p的过表达下调IPEC-J2细胞中IL-1β和TNFα的mRNA表达。

结论

我们的研究为 miRNA 在罗伊氏乳杆菌I5007 治疗后仔猪肠道功能中的作用提供了新的见解。