Considering that the genetically modified soybean lectin gene is affected by the gene type, to improve the stability of the genetically modified soybean lectin gene, a method based on fluorescence quantitative PCR to analyze the genetic characteristics of the genetically modified soybean lectin was proposed. The common soybean varieties, Wangshuibai and Huangdou No. 3 were selected as materials for tissue-specific expression analysis. Under the background conditions of analyzing the genetically modified soybean lectin genes, fluorescent quantitative PCR was applied to the analysis of genetic characteristics. The characteristics of the genetically modified soybean lectin gene were analyzed in terms of location characteristics and expression characteristics. The results showed that the soybean lectin gene has a complex functional mechanism and may participate in a variety of stress-related regulatory or signal transduction pathways in different ways; Lectin2.1 transcripts are expressed in abundance in glume and lemma in seedling tips, Lectin2.2 was mainly expressed in the roots, and a small amount was expressed in leaves and lemma; Lectin2.1 and Lectin2.2 are highly similar in nucleic acid and amino acid composition, and have similar subcellular localization characteristics.

鉴于转基因大豆凝集素基因受基因类型的影响，为提高转基因大豆凝集素基因的稳定性，提出了一种基于荧光定量PCR分析转基因大豆凝集素遗传特性的方法。选择常见的大豆品种王水白和黄豆3号作为组织特异性表达分析的材料。在分析转基因大豆凝集素基因的背景条件下，将荧光定量PCR技术应用于遗传特性分析。从定位特征和表达特征方面分析了转基因大豆凝集素基因的特征。结果表明，大豆凝集素基因具有复杂的功能机制，并可能以不同方式参与多种与压力相关的调控或信号转导途径。Lectin2.1转录本在幼苗尖端的颖花和外中大量表达，Lectin2.2主要在根中表达，少量在叶和内中表达；Lectin2.1和Lectin2.2在核酸和氨基酸组成上高度相似，并具有相似的亚细胞定位特性。