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Reduced Expression of C-class Genes Is Associated with the Multiple-petal Phenotype in Nelumbo nucifera
Horticulture Journal ( IF 1.2 ) Pub Date : 2020-10-30 , DOI: 10.2503/hortj.utd-214
Yumiko Kashiwamura 1 , Rikuto Matsuzawa 1 , Yusei Ishikawa 1 , Michio Shibata 1 , Yohei Higuchi 1
Affiliation  

The sacred lotus (Nelumbo nucifera) is an aquatic basal eudicot that is widely cultivated for food, medicine, and ornamental use. Ornamental lotus cultivars form flowers of various colors and shapes. The ABCE model for flower development has been proposed for model core eudicots such as Arabidopsis and snapdragon, but little is known about the molecular mechanisms of floral organ specification and development in N. nucifera. Here, we identified ABCE-class genes in the N. nucifera genome and compared their gene structures and spatial expression patterns in floral organs between single- and double-petalled flower-type lotus cultivars. Two lotus homologs of the C-class gene AGAMOUS (NnAG1, NnAG2) in a double-petal flower cultivar had intact transcripts, suggesting that loss of function of AG is not the cause of the double-petal phenotype in N. nucifera. NnAG1 and NnAG2 expression were specifically observed in stamens and carpels of a single-petalled flower cultivar, but were slightly reduced in the double-petalled flower cultivar. In situ hybridization analysis further revealed that these two C-class homologs are specifically expressed in inner whorls such as stamens and carpels in a single-petal flower cultivar, but the signals are diminished in double- and proliferating-petalled cultivars. These findings suggest that down-regulation of C-class genes is responsible for the multiple-petal phenotype in N. nucifera.



中文翻译:

C类基因的减少的表达与莲核中的多瓣表型有关。

神圣的莲花(Nelumbo nucifera)是一种水生的基础真杜鹃,广泛用于食品,药品和观赏用途。观赏莲​​花品种形成各种颜色和形状的花朵。已经提出了用于花发育的ABCE模型用于模式核心真双子叶植物,例如拟南芥和金鱼草,但是对N. nucifera的花器官规格和发育的分子机理知之甚少。在这里,我们在N. nucifera基因组中鉴定了ABCE类基因,并比较了它们的基因结构和单瓣和双瓣花型莲花品种在花器官中的空间表达模式。C级基因AGAMOUSNnAG1的两个荷花同源物NnAG2)在双瓣花品种中具有完整的转录本,这表明AG的功能丧失不是N. nucifera的双瓣表型的原因。NnAG1NnAG2的表达特别是在单瓣花品种的雄蕊和心皮中观察到,但在双瓣花品种的雄蕊和心皮中则有所降低。原位杂交分析进一步揭示了这两个C类同源物在单花瓣花卉品种的雄蕊和心皮等内旋中特异性表达,但在双花瓣和有花瓣繁殖品种中信号减弱。这些发现表明,C类基因的下调是造成N. nucifera中多瓣表型的原因。

更新日期:2020-12-28
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