The extensive use of preservatives during the growth, transport and storage of vegetables has been a concern because of their known or suspected toxicity that jeopardizes human health. This paper reports the development of a technique that rapidly determines the presence of five paraben preservative residues in leafy vegetables using double-vortex-assisted matrix solid-phase dispersion (DVA-MSPD) and UHPLC-electrospray ionization(−)-quadrupole time-of-flight mass spectrometry detection. We simplified the original MSPD technique by eliminating the use of mortar/pestle and SPE-column procedures. The DVA-MSPD factors were screened by a multilevel categorical design, and then optimized by Box–Behnken Design plus response surface methodology. The limits of quantification were 1.2–1.8 ng g⁻¹ (dry weight). The satisfactory average recoveries were 85–104% with RSDs less than 10%. The developed method was successfully employed for the rapid determination of selected paraben residues at trace-level in leafy vegetable samples.

中文翻译：

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双涡流辅助基质固相分散体快速测定叶类蔬菜中对羟基苯甲酸酯类防腐剂残留量的优化

在蔬菜的生长、运输和储存过程中广泛使用防腐剂一直受到关注，因为它们已知或怀疑具有危害人类健康的毒性。本文报道了一种使用双涡流辅助基质固相分散 (DVA-MSPD) 和 UHPLC-电喷雾电离 (−)-四极杆时间快速测定叶类蔬菜中是否存在五种对羟基苯甲酸酯防腐剂残留物的技术的开发。 -飞行质谱检测。我们通过消除研钵/研杵和 SPE 柱程序的使用简化了原始 MSPD 技术。DVA-MSPD因子通过多级分类设计进行筛选，然后通过Box-Behnken设计加上响应面方法进行优化。定量限为 1.2–1.8 ng g ⁻¹（干重）。令人满意的平均回收率为 85-104%，RSD 小于 10%。所开发的方法已成功用于快速测定叶类蔬菜样品中痕量水平的选定对羟基苯甲酸酯残留量。