Laser-assisted protein adsorption by photobleaching (LAPAP) is a versatile tool to nanopattern proteins on the micrometer scale. Sub-micron patterning is, however, difficult due to diffraction. We show that, similar to stimulated emission depletion (STED) microscopy, a depleting beam can effectively suppress LAPAP and hence is apt to locally control LAPAP in order to write sub-diffractional lines of proteins. Specifically, we attach biotinylated Atto 390 to glass substrates and incubate with Alexa 555 labeled streptavidin. The Alexa 555 is subsequently imaged with STED nanoscopy. The method is currently limited by diffusion of the biotinylated Atto 390 molecules.

中文翻译：

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STED控制的光致漂白，用于亚衍射光学纳米图案

通过光漂白（LAPAP）进行的激光辅助蛋白质吸附是一种用于在微米级纳米图案蛋白质的多功能工具。然而，由于衍射，亚微米图案化困难。我们显示，类似于激发发射耗尽（STED）显微镜，耗尽束可以有效抑制LAPAP，因此易于局部控制LAPAP，以编写蛋白质的亚衍射谱线。具体来说，我们将生物素化的Atto 390附着在玻璃基板上，并与Alexa 555标记的链霉亲和素一起孵育。随后使用STED纳米显微镜对Alexa 555进行成像。目前，该方法受到生物素化Atto 390分子扩散的限制。