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Cervical-Vaginal Microbiome and Associated Cytokine Profiles in a Prospective Study of HPV 16 Acquisition, Persistence, and Clearance
Frontiers in Cellular and Infection Microbiology ( IF 5.7 ) Pub Date : 2020-08-21 , DOI: 10.3389/fcimb.2020.569022
Anna-Barbara Moscicki 1 , Baochen Shi 2 , Hazel Huang 2 , Emma Barnard 2 , Huiying Li 2
Affiliation  

Persistent human papillomavirus (HPV) infections is necessary for the development of cervical cancers. Consequently, understanding the biologic mechanisms resulting in clearance is key in cancer prevention. Similar to other mucosal sites, it is expected that the local microbiome plays a significant role in shaping the immune response responsible for HPV clearance. Using cervical wash repository samples from a prospective study of HPV in women, this study investigates the microbiome and its associated inflammatory milieu during HPV 16 pre-acquisition, persistence and clearance states. For comparison, samples from women with no history of HPV ever during the study period were selected. We showed that 9 of 13 inflammatory cytokines were found to be significantly increased in the immediate post-clearance visit compared to the pre-acquisition or infection visits. Gardnerella vaginalis was associated with higher levels of inflammatory cytokines. Women with no history of HPV infection had similar cytokine profiles as those with HPV 16 post-clearance. This in vivo study documented an immune response shortly after HPV 16 clearance. G. vaginalis appeared to be involved in shaping this immune response. The appearance of G. vaginalis may have resulted from a shift from anti-microbial to anti-viral immune response with loss of bacterial control. The similar high levels of cytokines seen in women with no history of HPV suggest that a certain level of inflammatory surveillance is required to maintain an HPV negative state. This data may inform therapies such as probiotics or pro-inflammatory agents for treatment of persistent HPV.



中文翻译:

宫颈阴道微生物组和相关的细胞因子谱在HPV 16采集,持续性和清除的前瞻性研究中

持续性人类乳头瘤病毒(HPV)感染对于宫颈癌的发展是必要的。因此,了解导致清除的生物学机制是预防癌症的关键。与其他粘膜部位相似,预计局部微生物组在塑造负责HPV清除的免疫反应中起重要作用。本研究使用对女性HPV进行前瞻性研究的宫颈洗液储存库样本,研究了HPV 16预采集,持久性和清除状态期间的微生物组及其相关的炎症环境。为了进行比较,选择了在研究期间从未有过HPV病史的女性样本。阴道加德纳菌与较高水平的炎症细胞因子有关。没有HPV感染史的妇女与清除后HPV 16的妇女具有相似的细胞因子谱。这个体内 研究记录了HPV 16清除后不久的免疫反应。 阴道G.似乎参与了这种免疫反应的形成。外观阴道G.可能是由于从抗微生物免疫反应转向抗病毒免疫反应而失去了细菌控制。在没有HPV病史的女性中观察到的相似的高水平细胞因子表明,维持HPV阴性状态需要一定水平的炎症监测。该数据可为诸如益生菌或促炎剂等治疗持久性HPV的治疗提供信息。

更新日期:2020-09-25
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