Abstract

Purpose

The aim of this study was to evaluate the effects of oleuropein radiation protection and to find an effective radioprotector.

Materials and Method

Human mononuclear cells were treated with oleuropein at the concentration of 100 μM (optimum concentration), incubated for 24 h, and then exposed to 2 Gy gamma-rays. The anti-radiation effect of oleuropein was assessed by MTT assay, flow cytometry, comet assay, and micronucleus (MN) assay.

Results

It was found that pretreatment with oleuropein (25, 50, 75, 100, 200, 400, and 800 nM, and 1, 5, 10, 15, 20, 25, 30, 40, 50, 75, 100, 125, 150, 175, and 200 µM) significantly increased the percentage of cell viability compared to the irradiated group (p < .001). Moreover, oleuropein treatment with the above concentrations defined without gamma-ray did not show any cytotoxicity effect in human mononuclear cells. The LD_50/24h dose was calculated as 2.9 Gy, whereas by 200, 150, 50, and 100 µM oleuropein prior to radiation (1, 2,and 4 Gy), radiation LD_50/24h increased to 3.36, 3.54, 3.81, and >4 Gy, in that order. A very noticeable dose-modifying factor (DMF) of 1.16, 1.23, 1.31, and 1.72 was observed for 200, 150, 50, and 100 µM, in order. Therefore, 100 µM of oleuropein was selected as the desirable dose for radio-protection trial, and 2 Gy gamma-rays were used for further research. Human mononuclear cells treatment with oleuropein (100 µM) prior to 2 Gy gamma-rays significantly decreased apoptosis, genomic damage, and MN occurrence in human mononuclear caused by gamma-radiation (p < .001). Furthermore, treatment with oleuropein (100 µM) without radiation did not lead to apoptosis, genotoxicity, or clastogenic effects caused by oleuropein in human mononuclear cells.

Conclusion

The results revealed that oleuropein is able to significantly reduce cytotoxicity, apoptosis, genotoxic, and clastogenic effects of gamma-rays.

中文翻译：

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橄榄苦苷对培养的人淋巴细胞中辐射诱导的细胞毒性、细胞凋亡和遗传损伤的保护作用

摘要

目的

本研究的目的是评估橄榄苦苷辐射防护的效果并寻找有效的辐射防护剂。

材料和方法

人单核细胞用 100 μM（最佳浓度）橄榄苦苷处理，孵育 24 小时，然后暴露于 2 Gy 伽马射线。通过MTT法、流式细胞术、彗星法和微核（MN）法评估橄榄苦苷的抗辐射作用。

结果

发现用橄榄苦苷（25、50、75、100、200、400 和 800 nM，以及 1、5、10、15、20、25、30、40、50、75、100、125、 、175 和 200 µM) 与辐照组相比显着增加了细胞活力的百分比 ( p  < .001)。此外，在没有伽马射线的情况下用上述浓度的橄榄苦苷处理在人单核细胞中没有显示出任何细胞毒性作用。LD _50/24h剂量计算为 2.9 Gy，而在辐射前 200、150、50 和 100 µM 橄榄苦苷（1、2 和 4 Gy），辐射 LD _50/24h依次增加到 3.36、3.54、3.81 和 >4 Gy。对于 200、150、50 和 100 µM，按顺序观察到 1.16、1.23、1.31 和 1.72 的非常显着的剂量修正因子 (DMF)。因此，选择 100 µM 橄榄苦苷作为辐射防护试验的理想剂量，并使用 2 Gy 伽马射线进行进一步研究。在 2 Gy 伽马射线之前用橄榄苦苷 (100 µM) 处理人类单核细胞显着减少了由伽马辐射引起的人类单核细胞凋亡、基因组损伤和 MN 的发生 ( p  < .001)。此外，在没有辐射的情况下用橄榄苦苷 (100 µM) 处理不会导致橄榄苦苷在人单核细胞中引起的细胞凋亡、基因毒性或致裂体效应。

结论

结果表明，橄榄苦苷能够显着降低伽马射线的细胞毒性、细胞凋亡、遗传毒性和致细胞分裂作用。