ABSTRACT

Domestic, agricultural and industrial water activities lead to organic and inorganic pollution of the environment. Biotreatment of municipal wastewater with the potential production of biomass is a valuable feature of microalgae. In this study we evaluated the effects of wavelength and light intensity on phosphate and ammonium removal on the one hand, and biomass and protein production on the other hand by Spirulina platensis in municipal wastewater treatment under semi batch cultivation. S. platensis was inoculated at 40% in artificial wastewater open pond system. Red, blue and purple light with 3800, 4800 and 5800 lux light intensity under 12 h light and 12 h darkness were investigated. Cultivation was conducted in semi-batch conditions; after four days cultivation, one third of the culture was replaced with fresh medium. The highest biomass and protein concentrations were observed under blue light at 5800 lux light intensity, 5.45 and 3 g/l respectively cumulatively; while the highest amount of phosphate and ammonium removal were about 145 and 218 mg/l under purple light at 5800 lux intensity, respectively. The amounts of biomass and protein produced, as well as phosphate and ammonium removed, are therefore impacted by wavelength, light intensity, results show that light intensity and wavelength can be customized to reach on the one hand the highest biomass and protein production, and on the other hand to maximize the removal of phosphorous and ammonium.

摘要

家庭、农业和工业用水活动导致环境的有机和无机污染。具有潜在生物质生产的城市废水的生物处理是微藻的一个有价值的特征。在本研究中，我们一方面评估了波长和光强度对磷酸盐和铵去除的影响，另一方面评估了螺旋藻在半批量培养下的城市污水处理中的生物质和蛋白质生产。S. platensis在人工废水开放式池塘系统中接种 40%。研究了在 12 小时光照和 12 小时黑暗下具有 3800、4800 和 5800 勒克斯光强度的红光、蓝光和紫光。在半分批条件下进行培养；培养四天后，将三分之一的培养物更换为新鲜培养基。在 5800 勒克斯光强的蓝光下观察到最高的生物量和蛋白质浓度，累积分别为 5.45 和 3 g/l；而在 5800 勒克斯强度的紫光下，磷酸盐和铵的最高去除量分别约为 145 和 218 mg/l。因此，产生的生物质和蛋白质的量，以及去除的磷酸盐和铵盐，都会受到波长、光强度、