Rapid detection of biological agents in biodefense is critical for operational, tactical and strategic levels as well as for medical countermeasures. Yersinia pestis, Francisella tularensis, and Bacillus anthracis are high priority agents of biological warfare or bioterrorism and many response forces use lateral flow assays (LFAs) for their detection. Several companies produce these assays, which offer results in short time and are easy to use. Despite their importance, only few publications on the limits of detection (LOD) for LFAs are available. Most of these studies used inactivated bacteria or risk group‐2 strains. As the inactivation process in previous studies might have affected the tests’ performances, it was our aim in this study to determine and compare the LOD of several commercially available LFAs using viable risk group‐3 strains.

中文翻译：

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重新评估使用可行的第3组风险菌株检测鼠疫耶尔森氏菌，土拉弗朗西斯菌和炭疽芽孢杆菌孢子的12种侧向流免疫测定法的检测限

快速检测生物防御中的生物制剂对于运营，战术和战略层面以及医疗对策至关重要。鼠疫耶尔森氏菌，土拉弗朗西斯菌和炭疽杆菌是生物战或生物恐怖活动的重中之重，许多反应部队使用侧向流动分析（LFA）进行检测。几家公司生产这些测定法，可以在短时间内提供结果，并且易于使用。尽管它们很重要，但关于LFA的检测限（LOD）的出版物很少。这些研究大多数使用灭活细菌或危险组2菌株。由于以前的研究中的失活过程可能会影响测试的性能，因此本研究的目的是确定和比较使用第3类危险品菌株的几种市售LFA的LOD。