Pyridine, a highly toxic nitrogen-containing heterocyclic compound, is recalcitrant in the conventional biodegradation process. In this study, BiVO₄/FeOOH semiconductor-microbe interface was developed for enhanced visible-light-driven biodegradation of pyridine, where the efficiencies of pyridine removal (100%), total organic carbon (TOC) removal (88.06±3.76%) and NH₄⁺-N formation (84.51±8.95%) were remarkably improved, compared to the biodegradation system and photodegradation system. The electron transport system activity and photoelectrochemical analysis implied the significant improvement of photogenerated carriers transfer between microbes and semiconductors. High-throughput sequencing analysis suggested functional species related to pyridine biodegradation (Shewanella, Bacillus and Lysinibacillus) and electron transfer (Shewanella and Tissierella) were enriched at the semiconductor-microbe interface. The light-excited holes played a crucial role in promoting pyridine mineralization. This study demonstrated that this bio-photodegradation system would be a potential alternative for the efficient treatment of wastewater containing recalcitrant pollutant such as pyridine.

吡啶是一种剧毒的含氮杂环化合物，在常规的生物降解过程中是难降解的。在这项研究中，开发了BiVO ₄ / FeOOH半导体-微生物界面，以增强可见光驱动的吡啶生物降解，其中吡啶去除效率（100％），总有机碳（TOC）去除效率（88.06±3.76％）和与生物降解系统和光降解系统相比，NH ₄ ⁺ -N的形成（84.51±8.95％）显着改善。电子传输系统的活性和光电化学分析意味着光生载流子在微生物和半导体之间转移的显着改善。高通量测序分析表明与吡啶生物降解有关的功能物种（Shewanella，芽孢杆菌和Lysinibacillus）和电子转移（Shewanella和Tissierella）在半导体-微生物界面处富集。光激发的空穴在促进吡啶矿化中起关键作用。这项研究表明，这种生物光降解系统将成为有效处理含有难处理污染物（例如吡啶）的废水的潜在替代方法。