Although vitamin deficiencies are a well-known issue, monitoring of the vitamin status and diagnosis of vitamin deficiencies in remote regions is a complicated task. Dried blood microsampling, as an alternative sampling strategy, could offer a solution to the several drawbacks related to conventional venipuncture. Although highly relevant, the number of microsampling procedures that has been developed for assessing vitamins in dried blood samples is rather limited, indicating the challenging nature of the subject. This review discusses several challenges inherent to vitamin analysis in microsamples, thereby covering topics including sampling strategy, hematocrit (Hct) effect, sample preparation, calibration, assay sensitivity, stability, and clinical interpretation of the results.

尽管维生素缺乏症是一个众所周知的问题，但在偏远地区监测维生素状况和诊断维生素缺乏症是一项复杂的任务。干血微量采样作为一种可选的采样策略，可以解决与常规静脉穿刺相关的几个缺点。尽管高度相关，但是已经开发出的用于评估干血样品中维生素的微量采样程序的数量非常有限，这表明该受试者具有挑战性。这篇综述讨论了微量样品中维生素分析所固有的若干挑战，从而涵盖了主题，包括采样策略，血细胞比容（Hct）效果，样品制备，校准，测定灵敏度，稳定性和结果的临床解释。