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mtDNA genetic characterization of an isolated sheep breed in South of Moroccan Atlas
Small Ruminant Research ( IF 1.8 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.smallrumres.2020.106250
Asmae Kandoussi , Ismaïl Boujenane , Mohammed Piro , Daniel Petit

Abstract The present study aims to investigate the genetic variability of the Blanche de Montagne breed (=Siroua sheep). The genetic variability was assessed by the analysis of a 628 bp fragment in the mtDNA of the control region from 32 unrelated animals. Sequence comparison revealed 44 polymorphic sites, of which 16 were parsimony informative and 28 were singletons. Haplotype diversity and nucleotide diversity were 0.970 and 0.013, respectively, indicating a correct level of diversity. A total of 24 haplotypes were distinguished and phylogenetic analysis detected two maternal lineages (haplogroups B and C) with a predominance of haplogroup B. Neutrality tests indicated a demographic expansion of the breed in the past. Network analysis suggested a strong genetic structure of the breed and displayed a low level of inbreeding.

中文翻译:

摩洛哥阿特拉斯南部一个孤立绵羊品种的 mtDNA 遗传特征

摘要 本研究旨在调查 Blanche de Montagne 品种(= Siroua 羊)的遗传变异性。通过分析来自 32 只无关动物的对照区域的 mtDNA 中的 628 bp 片段来评估遗传变异性。序列比较揭示了 44 个多态位点,其中 16 个是简约信息,28 个是单例。单倍型多样性和核苷酸多样性分别为 0.970 和 0.013,表明多样性水平正确。总共区分了 24 个单倍型,系统发育分析检测到两个母系谱系(单倍群 B 和 C),其中单倍群 B 占优势。中性测试表明该品种过去有人口统计扩展。网络分析表明该品种具有很强的遗传结构,并显示出低水平的近亲繁殖。
更新日期:2020-12-01
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