Abstract A polarization-sensitive full-field optical coherence tomography (PS-FFOCT) system was developed, which is capable of revealing both the reflectivity and phase-retardation caused by the anisotropic samples simultaneously with the lateral resolution of 0.98 µm and the axial resolution of 2.4 µm. The system was used to generate depth-resolved en face isotropic intensity images and birefringent images of the normal and abnormal large intestine tissue samples of human in vitro. To our knowledge, for the first time, the intestine gland cells and intercellular structures of normal intestine and infiltration of metaplasia of cancerous intestine tissue were observed in the en face images obtained with PS-FFOCT device. The resolution and effects of measurement errors are discussed. The results obtained in this work demonstrated the potential applications of PS-FFOCT technique in the rapid intra-operation diagnosis of cancers.

中文翻译：

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使用偏振敏感 FFOCT 对大肠细胞间结构进行深度分辨成像

摘要 研制了偏振敏感全场光学相干断层扫描 (PS-FFOCT) 系统，能够同时揭示各向异性样品引起的反射率和相位延迟，横向分辨率为 0.98 µm，轴向分辨率为2.4 微米。该系统用于生成人体外正常和异常大肠组织样本的深度分辨正面各向同性强度图像和双折射图像。据我们所知，这是第一次在 PS-FFOCT 设备获得的正面图像中观察到正常肠道的肠腺细胞和细胞间结构以及癌变肠组织的化生浸润。讨论了测量误差的分辨率和影响。