Opisthorchis viverrini and other foodborne trematode infections are major health concerns in the Greater Mekong Subregion. Currently, the gold-standard diagnostic method for opisthorchiasis is conventional stool examination for the presence of parasite eggs. This method lacks sensitivity and needs an experienced technician. We therefore produced monoclonal antibodies to highly immunogenic O. viverrini proteins aiming at detecting specific antigens in the feces. In this study, BALB/C mice were immunized using semi-purified somatic antigens and spleen cells were fused with a Sp2/0 myeloma cell line. Four hybridomas (1A2, 1E12, 2C7 and 8D6) were selected and cloned due to their strong reaction against O. viverrini somatic protein, resulting in three IgM clones and one IgG2 clone. Immunohistochemistry showed that 1A2, 1E12, 2C7 and 8D6 stained the parenchyma cells, gut, tegument and muscles, respectively. Western-blot analysis revealed that only antibody 1A2 could detect coproantigen (approx. 73 kDa protein) in feces of hamsters infected with O. viverrini. The 1A2 monoclonal antibody may be of value in the diagnosis of opisthorchiasis by coproantigen detection.

大湄公河次区域的主要健康问题是维氏弧菌和其他食源性吸虫感染。目前，用于阿米巴气肿的金标准诊断方法是常规检查粪便中是否存在寄生虫卵。这种方法缺乏敏感性，需要经验丰富的技术人员。因此，我们生产了针对高免疫原性维氏曲霉蛋白的单克隆抗体，旨在检测粪便中的特定抗原。在这项研究中，使用半纯化的体细胞抗原对BALB / C小鼠进行免疫，并将脾细胞与Sp2 / 0骨髓瘤细胞系融合。选择了四个杂交瘤（1A2、1E12、2C7和8D6）并克隆，因为它们对O. viverrini有强烈反应体蛋白，产生三个IgM克隆和一个IgG2克隆。免疫组织化学显示1A2、1E12、2C7和8D6分别对实质细胞，肠，外皮和肌肉染色。Western-blot分析表明，只有抗体1A2才能在感染了O. viverrini的仓鼠粪便中检测到coproantigen（约73 kDa蛋白）。1A2单克隆抗体可能在通过辅助原抗原检测来诊断气胸病中具有价值。