Polydopamine nanoparticles (PDA NPs), among nature-inspired building materials, show special functions for biomedical systems and exploring PDA derived nanostructures for future developments is a fast growing field. Herein, we demonstrated the first evaluation of the PDA NPs for the electrochemical determination of lung cancer cells. In the presented study, PDA NPs were synthesized in a mild and cost-effective fashion by self-polymerization of dopamine in an alkaline environment. The structural and chemical characterizations clearly demonstrated the formation of PDA NPs with controllable size (130 nm), hence applied as a suitable material to functionalize the pencil graphite electrode (PGE) surface to construct a cytosensing nanoprobe. The ability of the developed sensor (PDA NPs/PGE) for label-free electrochemical A-549 lung cancer cells detection was investigated. The designed PDA NPs based cytosensor exhibited good biocompatibility and sensitivity for impedimetric diagnosis of A-549 cells in a wide linear range (1.0 × 10²–1.0 × 10⁵ cells mL⁻¹) with low detection limit (25 cells mL⁻¹). Furthermore, the developed bioassay has great potential as liquid biopsy for early cancer detection.

在自然界启发的建筑材料中，聚多巴胺纳米颗粒（PDA NPs）对生物医学系统显示出特殊的功能，探索PDA衍生的纳米结构用于未来发展是一个快速发展的领域。在这里，我们展示了对PDA NPs进行电化学测定肺癌细胞的首次评估。在本研究中，通过在碱性环境中多巴胺的自聚合，以温和且具有成本效益的方式合成了PDA NP。结构和化学特征清楚地证明了可控制大小（130 nm）的PDA NP的形成，因此被用作合适的材料来功能化铅笔状石墨电极（PGE）表面以构建细胞感应纳米探针。研究了开发的传感器（PDA NPs / PGE）用于无标记电化学A-549肺癌细胞检测的能力。设计的基于PDA NPs的细胞传感器在广泛的线性范围（1.0×10）中对A-549细胞的阻抗诊断具有良好的生物相容性和敏感性² –1.0×10 ^5个细胞mL ^-1），检测限低（25个细胞mL ^-1）。此外，发达的生物测定作为早期癌症检测的液体活检具有巨大的潜力。