Alkaline phosphatase (ALP) is an enzyme that actively plays a significant role in the various metabolic processes by transferring a phosphate group to the protein, nucleic acid, etc. The elevated level of ALP in blood plasma is the hallmark of inflammation/cancer. The hyperactive mitochondria in cancer cells produce an excess of ATP to fulfill the high energy demand. Thus, we have developed a fluorescent probe Mito-Phos for ALP, which can detect phosphatase expression in mitochondria in live cells. The probe Mito-Phos has shown ~15-fold fluorescence intensity increments at 450 nm in the presence of 500 ng/mL of ALP. It takes about 60 min to consume the whole amount of ALP (500 ng/mL) in physiological buffer saline. It can selectively react with ALP even in the presence of other probable cellular reactive components. It is highly biocompatible and nontoxic to the live cells. It has shown ALP expression in a dose-dependent manner by providing concomitant fluorescence images in the blue-channel region. It has localized exclusively in the mitochondria in live cells. The probe Mito-Phos is highly biocompatible with the ability to assess ALP expression in mitochondria in live cells.

碱性磷酸酶（ALP）是一种酶，通过将磷酸基团转移到蛋白质，核酸等上，在各种代谢过程中均起着重要作用。血浆中ALP的升高是炎症/癌症的标志。癌细胞中过度活跃的线粒体产生过量的ATP来满足高能量需求。因此，我们开发了一种用于ALP的荧光探针Mito-Phos，它可以检测活细胞中线粒体中的磷酸酶表达。探针Mito-Phos已经显示在500 ng / mL的ALP存在下，在450 nm处荧光强度增加约15倍。在生理缓冲盐水中消耗全部量的ALP（500 ng / mL）大约需要60分钟。即使在其他可能的细胞反应性成分存在下，它也可以选择性地与ALP反应。它具有高度的生物相容性，对活细胞无毒。通过在蓝通道区域提供伴随的荧光图像，它已经显示出ALP表达的剂量依赖性。它仅位于活细胞的线粒体中。Mito-Phos探针具有高度的生物相容性，能够评估活细胞中线粒体中的ALP表达。