Linalool is a monoterpene alcohol, which imparts floral scents to a variety of plants and is extensively used in various kinds of products, such as processed foods and beverages for fragrances and flavors. However, linalool from natural resources is racemate, and production of linalool enantiomers is difficult. To produce stereospecific linalool, we evaluated linalool synthase genes (LINS) from plants, such as Actinidia arguta (AaLINS) and Coriandrum sativum (CsLINS) for (S)-specific LINS and a gram-positive bacterium Streptomyces clavuligerus (ScLINS) for (R)-specific LINS, with Pantoea ananatis strain as the host. Among the 16 LINS examined, AaLINS and ScLINS showed the best (S)-linalool production and (R)-linalool production, respectively, with 100 % enantio excess. Co-expression of the mutated farnesyl diphosphate synthase gene, ispA* (S80 F), from Escherichia coli along with the LINS genes also improved linalool production. In order to prevent volatilization and cell toxicity of linalool, two-phase cultivation with isopropyl myristate was done, which had positive effects on linalool production. The carbon flux to the MVA pathway from glucose was increased by inactivating a membrane-bound glucose dehydrogenase. Overall, 5.60 g/L (S)-linalool and 3.71 g/L (R)-linalool were produced from 60.0 g/L glucose by introduction of AaLINS-ispA* and ScLINS-ispA* in P. ananatis, respectively.

芳樟醇是一种单萜醇，可赋予多种植物花香，并广泛用于各种产品，如加工食品和饮料的香料和调味剂。然而，来自自然资源的芳樟醇是外消旋体，并且芳樟醇对映体的生产是困难的。为了产生立体有择芳樟醇，我们评估芳樟醇合酶基因（LINS从植物），例如软枣猕猴桃（AaLINS）和芫荽（CsLINS）为（S） -特异性LINS和革兰氏阳性细菌小棒链霉菌（ScLINS）为（R ) 特定的LINS，与Pantoea ananatis菌株作为寄主。间的16个LINS检查，AaLINS和ScLINS显示出最好的（S）-linalool生产和（R）-linalool生产，分别用100％的对映体过量。突变的法尼基二磷酸合酶基因的共表达，ISPA *（S80 F），从大肠杆菌与沿LINS基因还提高了芳樟醇的产量。为了防止芳樟醇的挥发和细胞毒性，采用肉豆蔻酸异丙酯进行两相培养，对芳樟醇的生产有积极的影响。通过使膜结合葡萄糖脱氢酶失活，增加了从葡萄糖到 MVA 途径的碳通量。整体，5.60克/升（S）-linalool 3.71克/升（R）-linalool被60.0克/升葡萄糖通过引入的产生AaLINS - ISPA *和ScLINS - ISPA *在P.ananatis中，分别。