Addition effects of insulin-like growth factor-1 (IGF-1) and its synthetic analogue insulin-like growth factor-1 recombinant-3 (LongR3-IGF-1) after in vitro maturation (IVM) of cattle cumulus-oocyte complexes (COCs) were compared and evaluated on meiotic progression, apoptosis and profile genes of oocyte competence (GDF9, BMP15, BAX, BCL2, OOSP1, IGFBP2, IGBFP4 and IGFBP5), and their respective cumulus cells (AREG, EGFR, FSHR, COX2, BAX, BCL2, IGFBP2, IGFBP4 and IGFBP5). The 739 COCs (n = 10 pools) of bovine ovaries were collected, selected and matured with IGF-1 (100 ng/mL), LongR3-IGF-1 (100 ng/mL), and in two control groups with 0.1% polyvinyl alcohol (PVA) or 10% fetal bovine serum (FBS), for 22–24 h. The statistical analysis was performed by a linear mixed effects model, ANOVA and Tukey tests. There was no statistical difference between experimental groups taken into account the meiotic progression and apoptosis (P > 0.05). Nevertheless, there were statistical differences (P ≤ 0.05) among FBS, IGF-1 and LongR3-IGF-1 groups for IGFBP4 gene expression, and among PVA, IGF-1 and LongR3-IGF-1 for COX2 gene expression in cumulus cells. Moreover, statistical difference was found for BCL2 gene expression between IGF-1, FBS and PVA groups and for IGFBP4 gene expression between LongR3-IGF-1, PVA and FBS in oocytes. There was no statistical difference between experimental groups for other genes evaluated. These results showed a good performance of IVM of bovine oocytes in the presence of LongR3-IGF-1 and the possibility of replacement of IGF-1 and FBS.

牛卵丘卵母细胞复合物体外成熟 (IVM)后，胰岛素样生长因子-1 (IGF-1) 及其合成类似物胰岛素样生长因子-1 重组体-3 (LongR3-IGF-1) 的附加作用。COCs) 对减数分裂进程、凋亡和卵母细胞能力的谱基因 ( GDF9、BMP15、BAX、BCL2、OOSP1、IGFBP2、IGBFP4和IGFBP5 )及其各自的卵丘细胞 ( AREG、EGFR、FSHR、COX2、BAX )进行比较和评估, BCL2 ,IGFBP2、IGFBP4和IGFBP5 )。 收集、选择和成熟的 739个牛卵巢COC（n = 10 个池），使用 IGF-1 (100 ng/mL)、LongR3-IGF-1 (100 ng/mL)，并在两个对照组中使用 0.1% 聚乙烯酒精 (PVA) 或 10% 胎牛血清 (FBS)，22-24 小时。通过线性混合效应模型、ANOVA 和 Tukey 检验进行统计分析。考虑到减数分裂进程和细胞凋亡，实验组之间没有统计学差异（P  > 0.05）。尽管如此， FBS、IGF-1 和 LongR3-IGF-1 组之间的IGFBP4基因表达存在统计学差异（P ≤ 0.05），而 PVA、IGF-1 和 LongR3-IGF-1组之间的IGFBP4基因表达存在统计学差异（P ≤ 0.05）COX2基因在卵丘细胞中的表达。另外，统计学差异被发现的BCL2基因的表达IGF-1之间，FBS和PVA组和IGFBP4在卵母细胞LongR3-IGF-1，PVA和FBS之间的基因表达。对于评估的其他基因，实验组之间没有统计学差异。这些结果表明，在 LongR3-IGF-1 存在的情况下，牛卵母细胞的 IVM 性能良好，并且有可能替代 IGF-1 和 FBS。