Abstract Bacterial colonization on the surfaces of indwelling medical devices is one of the most important causes of related infections. By generating nano-vibration, the accumulation of bacterial colonies and the biofilm formation on the surface of materials can be hindered. In this paper, based on the adhesion characteristics between bacteria and the surface of materials, the antimicrobial mechanism of nano-vibration was revealed theoretically. By establishing the force model, which considered van der Waals force, electrostatic force, and hydrophobic force of Escherichia coli (E. coli) in the process of adhesion, we obtained the optimal amplitude range of nano-vibration to prevent E. coli attachment. Moreover, in vitro experiments were conducted to analyze the influence of the amplitude of nano-vibration on bacterial adhesion. The results showed that the aggregation of E. coli could be effectively avoided when the amplitude ranged from 0.3 nm to 3.8 nm, and the formation of bacterial biofilm could be successfully prevented when the amplitude of vibration on the surface was greater than 21 nm, which was in consistent with the theoretical analysis. This work provides a new reference for clinical prevention and treatment methods of indwelling device-related infections.

中文翻译：

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纳米振动有效防治材料上的大肠杆菌生物膜

摘要 留置医疗器械表面的细菌定植是相关感染的最重要原因之一。通过产生纳米振动，可以阻止细菌菌落的积累和材料表面生物膜的形成。本文基于细菌与材料表面的粘附特性，从理论上揭示了纳米振动的抗菌机理。通过建立考虑大肠杆菌粘附过程中范德华力、静电力和疏水力的力模型，我们得到了防止大肠杆菌粘附的最佳纳米振动振幅范围。此外，还进行了体外实验以分析纳米振动幅度对细菌粘附的影响。结果表明，振幅在0.3~3.8 nm范围内可以有效避免大肠杆菌的聚集，在表面振动振幅大于21 nm时，可以成功阻止细菌生物膜的形成。与理论分析一致。该工作为临床留置器械相关感染的防治方法提供了新的参考。