Benefit from the efficient energy transfer, aggregation-induced emission (AIE) and host-guest recognition as strategies of signal amplification and specific binding have been applied to establish the sensing system; however, the application of these two attractive strategies in one system is rare. Herein, we propose a “turn-on” to “turn-off” fluorescent strategy for sensitive detection of β-galactosidase (β-Gal) based on the application of AIE and host-guest recognition. In this work, a novel red-emitted (635 nm) copper nanoclusters (CuNCs) protected by dithioerythritol (DTE) and β-cyclodextrin (β-CD) has been found to possess AIE property induced by aluminum cations to achieve the “turn-on” process, and the coordinated behavior between aluminum cations and DTE/β-CD CuNCs is also discussed. As the hydrolysis product of β-Gal and 4-nitrophenyl-β-D-galactopyranoside, p-nitrophenol can combine with β-CD in DTE/β-CD CuNCs by the host-guest recognition to realize the efficient photoelectron transfer to “turn-off” the fluorescence. This strategy performs the exciting linear range of 0.0–50.0 U/L and detection limit of 0.56 U/L (S/N = 3) for sensitive detection of β-Gal and further applies in biologic samples successfully. As far as we know, this is the first work to combine AIE and host-guest recognition in one system to construct the sensing platform, which can achieve efficient and specific energy transfer to improve the sensitivity of the system.

由于信号放大和特异性结合的策略已被用于建立传感系统，因此得益于有效的能量转移，聚集诱导发射（AIE）和来宾识别。但是，这两种有吸引力的策略在一个系统中的应用很少。本文中，我们基于AIE和宿主客体识别的应用，提出了一种“开启”到“关闭”的荧光策略，用于灵敏地检测β-半乳糖苷酶（β-Gal）。在这项工作中，发现一种新的由二硫赤藓糖醇（DTE）和β-环糊精（β-CD）保护的红色（635 nm）铜纳米簇（CuNC）具有铝阳离子诱导的AIE特性，以实现“转角”。还讨论了铝阳离子与DTE /β-CDCuNCs之间的协同行为。对硝基苯酚是β-Gal和4-硝基苯基-β-D-吡喃半乳糖苷的水解产物，通过主客体识别，可以与DTE /β-CDCuNCs中的β-CD结合，实现有效的光电子转移。 -关闭”荧光。该策略可实现0.0-50.0 U / L的激发线性范围和0.56 U / L的检测极限（S / N = 3），以灵敏地检测β-Gal，并成功地应用于生物样品中。据我们所知，这是将AIE和来宾识别相结合在一个系统中构建传感平台的第一项工作，该传感平台可以实现高效而特定的能量传输，从而提高系统的灵敏度。该策略可实现0.0-50.0 U / L的激发线性范围和0.56 U / L的检测极限（S / N = 3），以灵敏地检测β-Gal，并成功地应用于生物样品中。据我们所知，这是将AIE和来宾识别相结合在一个系统中构建传感平台的第一项工作，该传感平台可以实现高效而特定的能量传输，从而提高系统的灵敏度。该策略可实现0.0-50.0 U / L的激发线性范围和0.56 U / L的检测极限（S / N = 3），以灵敏地检测β-Gal，并成功地应用于生物样品中。据我们所知，这是将AIE和来宾识别相结合在一个系统中构建传感平台的第一项工作，该传感平台可以实现高效而特定的能量传输，从而提高系统的灵敏度。