It has been shown earlier in a study of the role of the cytoskeletal protein zyxin in cell differentiation in the primordium of the central nervous system (CNS) of the spur-toed frog Xenopus laevis that zyxin interacts with three components of the signaling cascade of the secreted factor Sonic hedgehog (Shh): the transmembrane receptor Patched2 (Ptc2) and the transcription regulators Gli1 and Zic1. In the present work, the effect of zyxin knockdown on the expression of some key proteins of the Shh cascade has been studied. It has been shown using the RT-q PCR that the suppression of zyxin translation enhances the inhibitory effect of Shh on the expression of the target genes of this cascade such as Pax6, Irx3, and Dbx2 and affects the number of transcripts of several genes encoding the proteins that directly provide the functioning of the Shh cascade: Shh, Gli1, Ptc2, and Zic1. A hypothesis has been proposed that zyxin is involved in the regulation of the spatial marking of neural plate cells through the inhibitory effect on the Shh cascade.

中文翻译：

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细胞骨架蛋白 Zyxin 调节 Shh 信号级联靶基因在尖趾蛙非洲爪蟾胚胎神经板细胞中的表达

早先一项关于细胞骨架蛋白 zyxin 在尖趾蛙爪蟾中枢神经系统 (CNS) 原基细胞分化中作用的研究表明，zyxin 与爪蛙信号级联反应的三个组分相互作用。分泌因子声波刺猬 (Shh)：跨膜受体 Patched2 (Ptc2) 和转录调节因子 Gli1 和 Zic1。在目前的工作中，研究了 zyxin 敲低对 Shh 级联的一些关键蛋白表达的影响。使用 RT-q PCR 表明，抑制 zyxin 翻译增强了 Shh 对该级联靶基因表达的抑制作用，如 Pax6、Irx3、和 Dbx2 并影响编码直接提供 Shh 级联功能的蛋白质的几个基因的转录本数量：Shh、Gli1、Ptc2 和 Zic1。已经提出了一种假设，即 zyxin 通过对 Shh 级联的抑制作用参与神经板细胞空间标记的调节。