Biofilms on the tooth surface that are not removed regularly can lead to dental caries, gingivitis, or periodontal disease. Recently, numerous newly developed sterilization approaches and techniques based on nonthermal temperature gas plasma have been introduced in the medical field. This study investigates the influence of nitrogen-based nonthermal atmospheric pressure plasma jet (NTAPPJ) treatment on dental biofilms. To this end, oral biofilms were formed on saliva-coated hydroxyapatite (HA) disks in a batch culture at 37 °C under 5% CO₂. HA disks were treated with NTAPPJ for 0, 30, 60, and 120 s. The antibacterial efficacy of the NTAPPJ treatment was determined from the number of colony forming units (CFU). Morphology and structural changes in the bacteria were examined using scanning electron microscopy. The surface characteristics of the HA disks were analyzed with X-ray photoelectron spectroscopy (XPS) and contact angle measurements. CFU were reduced, and biofilm morphology and structure were significantly affected by plasma treatment. XPS and contact angle analysis showed that the surface of plasma-treated specimens was significantly richer in hydroxyl groups and more hydrophilic relative to untreated samples. There was no significant difference between the cytotoxicity of the treated and untreated samples for each test group. Therefore, these results show that atmospheric plasma treatment is very effective for biofilm release, which makes NTAPPJ a promising technique for various clinical dental applications, including bacterial sterilization.

不能定期清除的牙齿表面生物膜可能导致龋齿，牙龈炎或牙周疾病。最近，在医学领域已经引入了许多基于非热温度气体等离子体的新开发的灭菌方法和技术。这项研究调查了氮基非热常压等离子体射流（NTAPPJ）处理对牙齿生物膜的影响。为此，在37°C，5％CO ₂的分批培养中，在唾液包被的羟基磷灰石（HA）盘上形成口腔生物膜。。HA磁盘用NTAPPJ处理0、30、60和120 s。由菌落形成单位（CFU）的数量确定NTAPPJ处理的抗菌功效。使用扫描电子显微镜检查细菌的形态和结构变化。HA盘的表面特性用X射线光电子能谱（XPS）和接触角测量进行了分析。血浆处理降低了CFU，并且生物膜的形态和结构受到显着影响。XPS和接触角分析表明，与未经处理的样品相比，经等离子体处理的样品表面明显富含羟基，且亲水性更高。对于每个测试组，已处理和未处理样品的细胞毒性之间没有显着差异。因此，