In the present investigation, significant physical parameters such as incubation time, temperature and pH were optimized for cellulase production by wild and mutant strains of thermophilic Thermomyces dupontii. For a comparative analysis, three independent variables which were selected from OVAT were subjected to RSM. As per response surface methodology (RSM) model, central composite design was used in order to analyze the interactive effects of significant physical factors on cellulase productivity. The second polynomial regression model with a determination coefficient R² value 0.9503 and 0.9711 was found to be adequate and significant. 3D response surface plots and contours showed interaction among three variables. In contrast to wild T. dupontii, mutant strain showed 1.7-fold increase in FPase activity (43 U/ml/min) at 55 °C and pH 5.5 in 72 h. The result indicates thermophilic mutant T. dupontii has a potential for industrial use, because enzyme obtained from thermophile is more appropriate for industrial process where high temperature is involved.

在本研究中，重要的物理参数（如温育时间，温度和pH）针对嗜热嗜热霉菌的野生和突变菌株生产纤维素酶进行了优化。为了进行比较分析，对选自OVAT的三个自变量进行了RSM。根据响应面方法（RSM）模型，使用中央复合设计来分析重要物理因素对纤维素酶生产率的相互作用。确定系数R ²值为0.9503和0.9711的第二个多项式回归模型是足够的和有意义的。3D响应曲面图和轮廓线显示了三个变量之间的相互作用。与野生杜邦猪笼草相反突变菌株在55°C和pH 5.5下72小时内显示FPase活性（43 U / ml / min）增加1.7倍。结果表明，嗜热突变体杜邦螺旋体（T. dupontii）具有工业用途的潜力，因为从嗜热菌获得的酶更适合涉及高温的工业过程。