当前位置: X-MOL 学术Environ. Sci. Eur. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Multi-spatial contamination of environmental aquatic matrices with Cryptosporidium : a climate, health, and regulatory framework for the Philippines
Environmental Sciences Europe ( IF 5.9 ) Pub Date : 2020-09-25 , DOI: 10.1186/s12302-020-00410-w
Frederick R. Masangkay , Giovanni D. Milanez , Voravuth Somsak , Manas Kotepui , Jitbanjong Tangpong , Panagiotis Karanis

Background

Cryptosporidium is a waterborne global pathogen causing diarrhea primarily in infants and immunocompromised individuals. The Philippines is a tropical country susceptible to the influences of climate change and water crises. To date, the country has no existing epidemiologic data, regulation, or strategy for monitoring Cryptosporidium in freshwater systems. We, therefore, endeavored to provide evidence on the multi-spatial contamination of Cryptosporidium in environmental aquatic matrices using low-cost, user-friendly, and sustainable strategies and submit implications on the presence of Cryptosporidium in freshwater systems in a climate, health, and regulatory framework.

Results

Here, we present the microscopic detection of Cryptosporidium oocysts in low-volume (50 mL) environmental samples of surface water (SW), sediments (BW), and substrate-associated biofilm (SAB) and in 1 L bulk SW investigated by PCR. The multi-spatial distribution of Cryptosporidium oocysts in the low-volume (50 ml) aquatic matrices based on microscopy was highest at 69% (20/29) in SW and lowest at 50% (13/26) in BW. Immunofluorescence technique provided the highest microscopic positivity rate with 59% (17/29), 38% (10/26), and 50% (10/20) detection in SW, BW, and SAB, respectively. The detection and identification of Cryptosporidium in 1 L bulk SW by PCR and sequence analysis was recorded in total at 21% (6/29) in sampling sites where the differential identification of C. parvum, C. hominis, and Cryptosporidium spp. was 7% (2/29), 10% (3/29), and 3% (1/29), respectively.

Conclusions

We report the microscopical and first molecular epidemiologic data of Cryptosporidium from the most significant environmental freshwater systems in the Philippines. The presence of the two main human and animal pathogenic species C. parvum and C. hominis from the largest lakes and major water reservoirs in the country calls for sustainable solutions in safeguarding the quality of freshwater resources in a climate, health, and regulatory approach.



中文翻译:

隐孢子虫对环境水基质的多空间污染:菲律宾的气候,健康和法规框架

背景

隐孢子虫是一种水性全球病原体,主要在婴儿和免疫功能低下的人中引起腹泻。菲律宾是一个热带国家,容易受到气候变化和水危机的影响。迄今为止,该国尚无监测淡水系统中隐孢子虫的流行病学数据,法规或策略。因此,我们努力使用低成本,用户友好和可持续的策略,为环境水族基质中隐孢子虫的多空间污染提供证据,并就气候,健康和气候条件下淡水系统中隐孢子虫的存在提出影响。监管框架。

结果

在这里,我们介绍了隐孢子虫卵囊的微观检测,方法是在小体积(50 mL)地表水(SW),沉积物(BW)和与基质相关的生物膜(SAB)的环境样品中以及通过PCR研究的1 L散装SW中。根据显微镜检查,小体积(50 ml)水生基质中隐孢子虫卵囊的多空间分布在西南部最高,达到69%(20/29),而在BW中最低,达到50%(13/26)。免疫荧光技术提供了最高的显微镜阳性率,在SW,BW和SAB中分别检测到59%(17/29),38%(10/26)和50%(10/20)。隐孢子虫的检测与鉴定在1 L散装SW中,通过PCR和序列分析在采样点记录了总计21%(6/29)的差异,这些点在细小隐孢子虫,人形隐孢子虫隐孢子虫的鉴定中得到了鉴别。分别为7%(2/29),10%(3/29)和3%(1/29)。

结论

我们报告了来自菲律宾最重要的环境淡水系统的隐孢子虫的微观和第一分子流行病学数据。来自该国最大的湖泊和主要水库的两种主要的人类和动物病原体物种C. parvumC. hominis要求以可持续的解决方案,以气候,健康和监管的方式维护淡水资源的质量。

更新日期:2020-09-25
down
wechat
bug