Halogenated compounds are one of the largest groups of environmental-hazardous chemicals. The removal of the halogen atom from the substrate is possible by the catalytic activity of a type of enzyme called dehalogenase. Hydrolytic dehalogenases are suggested to be a good biodegradation catalyst for halogenated compounds with potential bioremediation applications. Therefore, the identification of possible bacterial strains that produce dehalogenase is of great importance. Soil microorganisms that are regularly exposed to halogenated pesticides are a major source of hydrolytic dehalogenase. Their proper identification may be useful in the production of high-quality dehalogenase. DNA stable isotope probing (DNA-SIP) is quite a useful technique for the identification of active microorganisms that assimilate specific carbon substrates and nutrients. Metagenomics combined with a stable isotope probe (SIP) technique could therefore be used to detect bacterial dehalogenases in pesticides exposed agricultural soil.

卤代化合物是对环境有害的化学物质中最大的一类。通过一种称为脱卤酶的酶的催化活性，可以从底物中去除卤素原子。水解脱卤酶被认为是具有潜在生物修复应用的卤代化合物的良好生物降解催化剂。因此，鉴定产生脱卤酶的可能菌株非常重要。经常接触卤化农药的土壤微生物是水解脱卤酶的主要来源。它们的正确鉴定可能有助于生产高质量的脱卤酶。DNA 稳定同位素探测 (DNA-SIP) 是一种非常有用的技术，用于识别同化特定碳底物和营养物质的活性微生物。