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A Study of Protein Fractional Composition during Incubation of Diphyllobothrium dendriticum (Cestoda) Plerocercoids in a Medium Containing Blood Serum of the Host, the Baikal Omul Coregonus migratorius (Coregonidae)
Biology Bulletin ( IF 0.5 ) Pub Date : 2020-09-24 , DOI: 10.1134/s1062359020040081
I. A. Kutyrev , O. B. Goreva , O. E. Mazur , V. A. Mordvinov

Abstract

The change in the composition of the protein fraction of Diphyllobothrium dendriticum (Cestoda) plerocercoids during incubation in a medium containing blood serum of their host—the Baikal omul Coregonus migratorius (Coregonidae)—was investigated. The change in the composition of the protein fraction of the incubation medium also was studied. On the one hand, the appearance of new high-molecular fractions with a molecular weight (MW) of 193 kDa after 3, 12, and 24 h of incubation and 88 kDa after 24 h was detected, which was associated at plerocercoids with the appearance of a new 189 kDa fraction after 12 h and the disappearance of the 80 kDa fraction. On the other hand, a decrease or the disappearance of two low-molecular fractions 57 and 42 kDa in the incubation medium after 3, 12, and 24 h of incubation were observed.



中文翻译:

在含有宿主血清贝加尔·奥穆尔·Coregonus migratorius(Coregonidae)血清的介质中的树突棘(Cestoda)皮囊菌孵化过程中蛋白质组分组成的研究

摘要

在的蛋白质级分的组合物中的变化裂头dendriticum(绦虫纲)plerocercoids孵育期间在培养基中含有的其血清主机贝加尔贝加尔白鲑白鲑migratorius(Coregonidae)-已进行调查。还研究了孵育培养基的蛋白质部分的组成变化。一方面,在孵育3、12和24 h后检测到分子量为193 kDa的新分子级分的出现,在24 h后检测到分子量为88 kDa的新分子级分,与拟除虫菊酯的出现有关12小时后,得到一个新的189 kDa馏分,而80 kDa馏分消失了。另一方面,在孵育3、12和24小时后,在孵育培养基中观察到两个低分子级分57和42kDa的减少或消失。

更新日期:2020-09-25
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