Abstract

This study was aimed at evaluating the neuroprotective effect of N-docosahexaenoyldopamine (DHA-DA), an endogenous bioactive lipid of the neurolipin family, on mesencephalic dopaminergic neurons of mouse fetuses in a cell culture under exposure to 1-methyl-4-phenylpyridinium⁺ (MPP⁺), a specific neurotoxin for catecholaminergic neurons. The state of neurons in the experiment (MPP⁺) and in the control (0.9% NaCl) was estimated according to three parameters: the number of surviving dopaminergic neurons, the length of the neurites, and the total content of dopamine in the neurons. DHA-DA was shown to have a dose-dependent neuroprotective effect on degenerating dopaminergic neurons under the influence of MPP⁺. DHA-DA at concentrations of 0.5 μM or lower does not have a neuroprotective effect. However, at higher concentrations it has a neuroprotective effect, preventing the death of dopaminergic neurons and the degradation of neuritis. At a concentration of 1 μM, DHA-DA protects the neuron cell bodies from degradation to a higher extent than their processes, whereas at a concentration 2 μM it protects and/or stimulates to a greater extent the growth of processes of the surviving neurons. In addition, DHA-DA reduces significantly the loss of dopamine in neurons under exposure to MPP⁺. Thus, it is shown that DHA-DA has a dose-dependent neuroprotective effect on degenerating dopaminergic neurons.

中文翻译：

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N-二十二碳六烯酰多巴胺对中脑退化多巴胺能神经元的神经保护作用

摘要

这项研究旨在评估N-二十二碳六烯酰多巴胺（DHA-DA）（一种神经脂家族的内源性生物活性脂质）对暴露于1-甲基-4-苯基吡啶鎓⁺的细胞培养物中小鼠胎儿的中脑多巴胺能神经元的神经保护作用。（MPP ⁺），一种儿茶酚胺能神经元的特定神经毒素。根据三个参数估算实验中（MPP ⁺）和对照组（0.9％NaCl）中神经元的状态：存活的多巴胺能神经元数量，神经突长度和神经元中多巴胺的总含量。在MPP ⁺的影响下，DHA-DA对退化的多巴胺能神经元具有剂量依赖性的神经保护作用。。浓度为0.5μM或更低的DHA-DA没有神经保护作用。但是，在较高浓度下，它具有神经保护作用，可防止多巴胺能神经元的死亡和神经炎的退化。在浓度为1μM时，DHA-DA保护神经元细胞体的降解程度高于其过程，而在浓度为2μM时，DHA-DA保护和/或更大程度地保护存活的神经元过程的生长。此外，DHA-DA显着降低了MPP ⁺暴露下神经元中多巴胺的损失。因此，表明DHA-DA对退化的多巴胺能神经元具有剂量依赖性的神经保护作用。