Plant Disease ( IF 4.5 ) Pub Date : 2021-03-01 , DOI: 10.1094/pdis-08-20-1735-sc P M Dracatos 1 , R F Park 1 , D Singh 1
Improving resistance to barley leaf rust (caused by Puccinia hordei) is an important breeding objective in most barley growing regions worldwide. The development and subsequent utilization of high-throughput PCR-based codominant molecular markers remains an effective approach to select genotypes with multiple effective resistance genes, permitting efficient gene deployment and stewardship. The genes Rph20 and Rph24 confer widely effective adult plant resistance (APR) to leaf rust, are common in European and Australian barley germplasm (often in combination), and act interactively to confer high levels of resistance. Here we report on the development and validation of codominant insertion-deletion (indel) based PCR markers that are highly predictive for the resistance alleles Rph20.ai and Rph24.an (both referred to as Rph20 and Rph24).
中文翻译:
验证大麦叶锈病抗性基因Rph20和Rph24的分子标记。
提高抗大麦叶锈病(由小麦大麦)是全球最大麦产区的重要育种目标。高通量基于PCR的显性分子标记的开发和后续利用仍然是一种有效的方法,可以选择具有多个有效抗性基因的基因型,从而实现有效的基因部署和管理。Rph20和Rph24基因赋予广泛有效的成年植物对叶锈病的抗性(APR),在欧洲和澳大利亚的大麦种质中很常见(通常是结合使用),并且可以交互作用以赋予高水平的抗性。在这里,我们报告基于共插入-缺失(indel)的PCR标记的开发和验证,这些标记可高度预测抗性等位基因Rph20.ai和Rph24.an(均称为Rph20和Rph24)。