Microfluidics has revolutionized several research areas by providing compact yet powerful microanalytical devices that in many cases outperform conventional systems. Among different microfluidics technologies, droplet microfluidics has emerged as a powerful platform to enable analyses of biological samples and phenomena because of its simplicity and versatility. Droplet microfluidics enables high-throughput encapsulation, manipulation, and analysis of single cells while drastically reducing the cost and time required by conventional technologies. For many of these microanalysis systems, manipulation of individual droplets is extremely important as it enables multiplexed high dimensional phenotyping of the targets, going beyond surface phenotyping. One of the key manipulation steps that needs to be implemented with high precision is enabling long-term observation of droplets and recovery of a subset of these droplets for further analysis. This Perspective highlights the recent advances and provides an outlook on future developments that will enable highly complex analyses of biological samples.

中文翻译：

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液滴的静态阵列和按需回收的生物学检测方法。

微流体技术通过提供紧凑但功能强大的微分析设备在许多情况下都优于常规系统，从而彻底改变了几个研究领域。在不同的微流控技术中，液滴微流控技术因其简单性和多功能性而成为一种强大的平台，可用于分析生物样本和现象。液滴微流控技术可实现单个细胞的高通量封装，操作和分析，同时大大降低了常规技术所需的成本和时间。对于许多此类微分析系统而言，对单个液滴的操作极为重要，因为它可以实现靶标的多重高维表型，而不仅仅是表面表型。需要高精度执行的关键操作步骤之一是能够长期观察液滴并回收这些液滴的子集以进行进一步分析。本《观点》重点介绍了最近的进展，并对未来的发展提供了前景，这将使生物样品的分析变得非常复杂。