As an oncogene, plasmacytoma variant translocation 1 (PVT1) has been found to be highly expressed in several cancers. However, its specific role in lung adenocarcinoma (ADC) has not been fully elucidated. In this study, the expression of PVT1, miR-378c, and solute carrier family 2 member 1 (SLC2A1) was determined by quantitative real-time PCR and western blot. Dual-luciferase reporter assay was used to explore the relationship between PVT1 and miR-378c, as well as miR-378c and SLC2A1. The effects of PVT1 on the lung ADC cells proliferation, invasion, and migration were detected using MTT, wound-healing, and transwell assays. The results revealed that PVT1 was highly expressed in lung ADC cells, and the overexpression of PVT1 promoted the proliferation, migration, and invasion of lung ADC cells. In lung ADC cells, PVT1 negatively regulated miR-378c expression, and miR-378c negatively regulated SLC2A1 expression through binding to its 3'-untranslated coding regions. Knocking down of PVT1 inhibited the abilities of cell proliferation, migration, and invasion, while miR-378c inhibitor or SLC2A1 Vector diminished the effect. Together, silencing PVT1 downregulated SLC2A1 expression via targeting miR-378c, and then repressed lung ADC cells growth, migration, and invasion.

中文翻译：

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lncRNA PVT1通过靶向miR-378c调节SLC2A1表达促进肺腺癌细胞的侵袭性生长。

作为一种致癌基因，浆细胞瘤变异易位 1 (PVT1) 已被发现在几种癌症中高度表达。然而，其在肺腺癌（ADC）中的具体作用尚未完全阐明。在本研究中，PVT1、miR-378c 和溶质载体家族 2 成员 1 (SLC2A1) 的表达通过实时定量 PCR 和蛋白质印迹法测定。双荧光素酶报告基因检测用于探讨 PVT1 与 miR-378c 以及 miR-378c 与 SLC2A1 之间的关系。使用 MTT、伤口愈合和 Transwell 试验检测 PVT1 对肺 ADC 细胞增殖、侵袭和迁移的影响。结果表明，PVT1在肺ADC细胞中高表达，PVT1的过表达促进了肺ADC细胞的增殖、迁移和侵袭。在肺 ADC 细胞中，PVT1 负调控 miR-378c 的表达，而 miR-378c 通过与其 3' 非翻译编码区的结合来负调控 SLC2A1 的表达。敲低 PVT1 会抑制细胞增殖、迁移和侵袭的能力，而 miR-378c 抑制剂或 SLC2A1 Vector 则减弱了这种作用。总之，沉默 PVT1 通过靶向 miR-378c 下调 SLC2A1 表达，然后抑制肺 ADC 细胞的生长、迁移和侵袭。