A small percentage of the total freshwater on Earth is represented by river water. Microbes have an essential role to play in the biogeochemical cycles, mineralization of organic water, along with xenobiotics degradation. Microbial dynamics are susceptible to environmental stressors which includes pollutants such as antibiotics, metals, and other degradants. River Yamuna is polluted extensively by domestic and industrial wastes. Xenobiotics, when released into the environment, can lead to water pollution. The present study evaluates the microbial diversity in Yamuna River (28°40′5.53′′ N, 77°15′0.35′′ E) along with the prediction of the metagenome function. In this context, the metagenomic DNA was extracted and sequencing was done on Illumina@MiSeq platform. The total number of OTUs picked was 41,994, out of which 74% were classified within the kingdom Bacteria. 35% of the OTUs were assigned to phylum Proteobacteria , followed by Bacteriodetes (22%), whereas 26% of OTUs were unassigned. PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was used to predict metagenomic functions using 16S rDNA as a marker. Metagenomic reads were assigned to the Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous (COG), and Gene Ontology (GO). Functional characterization reveals the presence of methyl-accepting chemotaxis protein which is an important adaptation for the microbes in the environment. The enzymes can be mapped for the bioremediation of xenobiotics. Information obtained from the amplicon sequencing of River Yamuna, collaborated with “omic” studies, may help in the design of bioremediation strategies and can be used for environmental clean-up of pollutants.

中文翻译：

---

使用 Illumina MiSeq 测序评估亚穆纳河的细菌多样性和功能概况

地球上淡水总量的一小部分是河水。微生物在生物地球化学循环、有机水矿化以及异生物质降解中发挥着重要作用。微生物动力学易受环境压力因素的影响，其中包括抗生素、金属和其他降解物等污染物。亚穆纳河被生活和工业废物广泛污染。外源性物质释放到环境中时，会导致水污染。本研究评估了亚穆纳河（28°40'5.53'' N，77°15'0.35'' E）的微生物多样性以及宏基因组功能的预测。在这种情况下，提取宏基因组 DNA 并在 Illumina@MiSeq 平台上进行测序。选取的 OTU 总数为 41,994，其中 74% 属于细菌界。35% 的 OTU 被分配给变形菌门，其次是拟杆菌 (22%)，而 26% 的 OTU 未分配。PICRUSt（通过重建未观察到的状态对社区进行系统发育调查）用于使用 16S rDNA 作为标记预测宏基因组功能。宏基因组读数被分配到京都基因和基因组百科全书 (KEGG)、直系同源 (COG) 和基因本体论 (GO)。功能表征揭示了甲基接受趋化蛋白的存在，这是对环境中微生物的重要适应。这些酶可以被定位用于异生物质的生物修复。从亚穆纳河的扩增子测序中获得的信息，与“组学”研究合作，