Microvesicles (MVs) are found in several types of body fluids and are promising disease biomarkers and therapeutic targets. This study aimed to develop a novel biofunctionalized surface for binding plasma microvesicles (PMVs) based on a lab-on-a-chip (LOC) approach. A new lactadherin (LACT)-functionalized surface was prepared and examined for monitoring PMVs. Moreover, two different strategies of LACT immobilization on a silicon surface were applied to compare different LACT orientations. A higher PMV to LACT binding efficiency was observed for LACT bonded to an αvβ3 integrin–functionalized surface compared with that for LACT directly bonded to a glutaraldehyde-modified surface. Effective binding of PMVs and its components for both LACT immobilization strategies was confirmed using spectral ellipsometry and time-of-flight secondary ion mass spectrometry methods. The proposed PMV capturing system can be used as a foundation to design novel point-of-care (POC) diagnostic devices to detect and characterize PMVs in clinical samples.

微囊泡（MVs）存在于几种体液中，是有前途的疾病生物标志物和治疗靶标。这项研究旨在基于芯片实验室（LOC）方法开发一种新颖的生物功能化表面，用于结合血浆微泡（PMV）。制备了新的内分泌粘附素（LACT）功能化表面，并进行了检测以监测PMV。此外，将两种不同的LACT固定在硅表面的策略用于比较不同的LACT方向。与直接结合到戊二醛修饰的表面的LACT相比，与αvβ3整联蛋白官能化的表面结合的LACT的PMV与LACT的结合效率更高。使用光谱椭圆偏振法和飞行时间二次离子质谱法，证实了PMV及其组分对LACT固定策略的有效结合。提出的PMV捕获系统可以用作设计新颖的即时诊断（POC）诊断设备的基础，以检测和表征临床样品中的PMV。