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Extracellular Vesicles Isolated from Mesenchymal Stromal Cells Primed with Hypoxia: Novel Strategy in Regenerative Medicine
Current Stem Cell Research & Therapy ( IF 2.7 ) Pub Date : 2021-03-31 , DOI: 10.2174/1574888x15999200918110638
Shalmali Pendse 1 , Vaijayanti Kale 1 , Anuradha Vaidya 1
Affiliation  

Mesenchymal stromal cells (MSCs) regulate other cell types through a strong paracrine component called the secretome, comprising several bioactive entities. The composition of the MSCs’ secretome is dependent upon the microenvironment in which they thrive, and hence, it could be altered by pre-conditioning the MSCs during in vitro culture. The primary aim of this review is to discuss various strategies that are being used for the pre-conditioning of MSCs, also known as “priming of MSCs”, in the context of improving their therapeutic potential. Several studies have underscored the importance of extracellular vesicles (EVs) derived from primed MSCs in improving their efficacy for the treatment of various diseases. We have previously shown that co-culturing hematopoietic stem cells (HSCs) with hypoxia-primed MSCs improves their engraftment potential. Now the question we pose is, would priming of MSCs with hypoxia favorably alter their secretome? and would this altered secretome work as effectively as the cell to cell contact did? Here we review the current strategies of using the secretome, specifically the EVs (microvesicles and exosomes), collected from the primed MSCs with the intention of expanding HSCs ex vivo. We speculate that effective priming of MSCs in vitro could modulate the molecular profile of their secretome, which could eventually be used as a cell-free biologic in clinical settings.



中文翻译:

从缺氧引发的间充质基质细胞中分离出的细胞外囊泡:再生医学的新策略

间充质基质细胞 (MSCs) 通过称为分泌组的强大旁分泌成分调节其他细胞类型,该成分包含多种生物活性实体。MSCs 分泌组的组成取决于它们茁壮成长的微环境,因此,它可以通过在体外培养期间对 MSCs 进行预处理来改变。本综述的主要目的是讨论用于 MSCs 预处理的各种策略,也称为“MSCs 启动”,以提高其治疗潜力。几项研究强调了源自致敏 MSC 的细胞外囊泡 (EV) 在提高其治疗各种疾病的功效方面的重要性。我们之前已经表明,将造血干细胞 (HSC) 与缺氧引发的 MSC 共培养可提高其植入潜力。现在我们提出的问题是,用缺氧启动 MSC 是否会有利地改变它们的分泌组?这种改变的分泌组会像细胞与细胞接触那样有效吗?在这里,我们回顾了当前使用分泌组的策略,特别是从准备好的 MSC 收集的 EV(微泡和外泌体),目的是体外扩增 HSC。我们推测体外 MSCs 的有效启动可以调节其分泌组的分子谱,最终可以在临床环境中用作无细胞生物制剂。从准备好的 MSC 中收集,目的是在体外扩增 HSC。我们推测体外 MSCs 的有效启动可以调节其分泌组的分子谱,最终可以在临床环境中用作无细胞生物制剂。从准备好的 MSC 中收集,目的是在体外扩增 HSC。我们推测体外 MSCs 的有效启动可以调节其分泌组的分子谱,最终可以在临床环境中用作无细胞生物制剂。

更新日期:2021-04-16
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