Severe inflammation can lead to multiple organ dysfunction syndrome, which has high mortality. Adipose-derived stem cells have been shown to affect the inflammatory response of macrophages. However, the molecular mechanism of the anti-inflammatory capacity of adipose-derived stem cells (ADSCs) remains to be understood. In the present study, a macrophage inflammation model was established by LPS, and treated with different volumes of ADSC supernatant. Then, we investigated the key genes in the LPS group and treatment group by RT-PCR, RNA sequencing technology, and bioinformatics analysis. A total of 26 miRNAs and 11,882 mRNAs were differentially expressed between them. The expression of 15 of the miRNAs (9 upregulated and 6 downregulated) was confirmed by RT-PCR. GO and KEGG pathway analyses of the targets of the 9 significantly upregulated miRNAs showed that they were related to immune system process, inflammatory response, lipopolysaccharide, and TNF-α, NF-κB, Toll-like receptor, and MAPK signaling pathways. Moreover, a miRNA-mRNA network also revealed 8 important genes (Mapkapk2, Sepp1, Cers6, Snn, ZfP568, Ccdc93, Pofut1, Pik3cd). We finally confirmed the expression of these 8 targeted genes by performing the RT-PCR analysis. This study may provide a new understanding of the molecular mechanism of ADSCs in the inflammatory response related to multiple miRNAs and mRNAs.

中文翻译：

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基于脂肪组织干细胞疗法的 LPS 诱导的巨噬细胞炎症中微 RNA 和 mRNA 的综合分析。

严重的炎症会导致多器官功能障碍综合征，死亡率很高。脂肪来源的干细胞已被证明会影响巨噬细胞的炎症反应。然而，脂肪干细胞（ADSCs）抗炎能力的分子机制仍有待了解。在本研究中，通过 LPS 建立巨噬细胞炎症模型，并用不同体积的 ADSC 上清液处理。然后，我们通过RT-PCR、RNA测序技术和生物信息学分析研究了LPS组和治疗组的关键基因。共有26个miRNA和11,882个mRNA在它们之间差异表达。RT-PCR 证实了 15 个 miRNA（9 个上调和 6 个下调）的表达。对 9 个显着上调 miRNA 的靶点进行 GO 和 KEGG 通路分析表明，它们与免疫系统过程、炎症反应、脂多糖以及 TNF-α、NF-κB、Toll 样受体和 MAPK 信号通路有关。此外，miRNA-mRNA 网络还揭示了 8 个重要基因（Mapkapk2、Sepp1、Cers6、Snn、ZfP568、Ccdc93、Pofut1、Pik3cd）。我们最终通过进行 RT-PCR 分析确认了这 8 个目标基因的表达。该研究可能为ADSCs参与多种miRNAs和mRNAs相关炎症反应的分子机制提供新的认识。Sepp1、Cers6、Snn、ZfP568、Ccdc93、Pofut1、Pik3cd）。我们最终通过进行 RT-PCR 分析确认了这 8 个目标基因的表达。该研究可能为ADSCs参与多种miRNAs和mRNAs相关炎症反应的分子机制提供新的认识。Sepp1、Cers6、Snn、ZfP568、Ccdc93、Pofut1、Pik3cd）。我们最终通过进行 RT-PCR 分析确认了这 8 个目标基因的表达。该研究可能为ADSCs参与多种miRNAs和mRNAs相关炎症反应的分子机制提供新的认识。