Leaf rust, caused by Puccinia triticina, is one of the most common wheat (Triticum aestivum) diseases in the Great Plains of the United States. A population of recombinant inbred lines from CI 17884 × ‘Bainong 418’ was evaluated for responses to leaf rust race Pt52-2 and genotyped using single nucleotide polymorphism (SNP) markers. Quantitative trait locus analysis identified a minor gene for resistance to leaf rust, designated QLr.stars-1RS, on the 1BL.1RS translocation segment in ‘Bainong 418’, and another leaf rust resistance gene, Lr47, on chromosome 7A of CI 17884. Lr47, originally identified in CI 17884 and located in a wheat-T. speltoides translocation segment 7S#1S, remains one of only a few race-specific resistance genes still effective in the Great Plains. A set of 7A-specific simple sequence repeat markers were developed and used to genotype CI 17884 and a pair of near-isogenic lines differing in the presence or absence of 7S#1S, PI 603918, and ‘Pavon F76’. Haplotype analysis indicated that the estimated length of 7S#1S was 157.23 to 174.42 Megabases, accounting for ∼23% of the 7A chromosome. Two SNPs on 7S#1S and four SNPs on the 1RS chromosome arm were converted to Kompetitive allele-specific PCR (KASP) markers, which were subsequently validated in a panel of cultivars and elite breeding lines released within the last decade. Of these, one- and two-KASP markers are specific to the 1RS chromosome arm and 7S#1S, respectively, indicating that they can facilitate the introgression of Lr47 and QLr.stars-1RS into locally adapted wheat cultivars and breeding lines.

由小麦锈菌引起的叶锈病是美国大平原地区最常见的小麦（Triticum aestivum）疾病之一。的重组自交系从CI 17884的群ד百农418”进行评价，以便回复到叶锈菌PT52 - 2，并使用单核苷酸多态性（SNP）标记物基因分型。数量性状基因座分析确定了一个抗叶锈病的次要基因，命名为QLr。分- 1RS，对“百农418”的1BL.1RS易位段，而另一个叶锈病抗性基因，Lr47，关于CI 17884.的染色体7A Lr47，最初在CI 17884中鉴定，位于小麦T型中。斯佩尔托德斯易位区段7S＃1S仍然是在大平原仍然有效的少数种族特异性抗性基因之一。已开发出一套7A特异性简单序列重复标记，并用于对CI 17884和一对在存在或不存在7S＃1S，PI 603918和'Pavon F76'方面有所不同的近等基因系进行基因分型。单倍型分析表明，估计的7S＃1S长度为157.23至174.42兆碱基，约占7A染色体的23％。将7S＃1S上的两个SNP和1RS染色体上的四个SNP转换为竞争竞争性等位基因特异性PCR（KASP）标记，随后在最近十年内发布的一组品种和优良育种系中对其进行了验证。其中，一个和两个KASP标记分别针对1RS染色体臂和7S＃1S，Lr47和QLr。星-将1RS导入当地适应的小麦品种和育种系。