Immobilization of the Geotrichum candidum (CCT 1205) cell with functionalized silica creates promising biocatalysts for production of ɛ-caprolactone. The results obtained by immobilization of the whole cell on SiO2-NH2 and SiO2-SH supports indicate that the presence of reactive functional groups on the support may promote effective chemical bonds with the cell walls resulting the decreased dehydrogenases enzyme activity (5% yield in less than 2h) and consequently, increased Baeyer-Villiger monooxygenases enzyme activity with redacting of 25% of time reaction when is used SiO2-NH2 as support and 50% through use of SiO2-SH as support relative to free cells when cyclohexanone is used as a substrate. The catalysts SiO2-NH2-Geotrichum candidum and SiO2-SH-Geotrichum candidum were recycling and reused in the ɛ-caprolactone synthesis from cyclohexanone, and the biocatalysts promoted a quantitative conversion up to the eighth reaction cycle. KEY POINTS: • Immobilized microorganism is more efficient than free cell in the caprolactone synthesis. • The reaction times for amino and thiol groups in support were 3 h and 2 h, respectively. • These catalysts showed higher ɛ-caprolactone conversion at higher concentrations.

中文翻译：

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利用固定在功能化二氧化硅上的念珠菌细胞生物催化生产ε-己内酯。

用功能化的二氧化硅固定白念珠菌（CCT 1205）细胞产生了生产β-己内酯的有前途的生物催化剂。通过将整个细胞固定在SiO2-NH2和SiO2-SH载体上而获得的结果表明，载体上反应性官能团的存在可以促进与细胞壁的有效化学键合，从而导致脱氢酶活性降低（在超过2h），因此，以SiO2-NH2为载体时，Baeyer-Villiger单加氧酶的酶活性增加了25％的时间反应，而以环己酮作为游离细胞时，相对于游离细胞，通过使用SiO2-SH为50％。基质。循环使用SiO2-NH2-Geotrichum candidum和SiO2-SH-Geotrichum candidum催化剂，并将其重新用于由环己酮合成ε-己内酯的过程中，直到第八个反应周期，生物催化剂都促进了定量转化。要点：•在己内酯合成中，固定化微生物比游离细胞更有效。•载体中氨基和巯基的反应时​​间分别为3小时和2小时。•这些催化剂在较高浓度下表现出较高的ε-己内酯转化率。