High-dose radiation exposure results in organ-specific sequelae that occurs in a time- and dose-dependent manner. The partial body irradiation with minimal bone marrow sparing model was developed to mimic intentional or accidental radiation exposures in humans where bone marrow sparing is likely and permits the concurrent analysis of coincident short- and long-term damage to organ systems. To help inform on the natural history of the radiation-induced injury of the partial body irradiation model, we quantitatively profiled the plasma proteome of non-human primates following 12 Gy partial body irradiation with 2.5% bone marrow sparing with 6 MV LINAC-derived photons at 0.80 Gy min over a time period of 3 wk. The plasma proteome was analyzed by liquid chromatography-tandem mass spectrometry. A number of trends were identified in the proteomic data including pronounced protein changes as well as protein changes that were consistently upregulated or downregulated at all time points and dose levels interrogated. Pathway and gene ontology analysis were performed; bioinformatic analysis revealed significant pathway and biological process perturbations post high-dose irradiation and shed light on underlying mechanisms of radiation damage. Additionally, proteins were identified that had the greatest potential to serve as biomarkers for radiation exposure.

中文翻译：

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非人类灵长类动物血浆的蛋白质组学在部分身体辐射后与最小的骨髓保留。

高剂量辐射暴露会导致器官特异性后遗症，这种后遗症以时间和剂量依赖性方式发生。开发了具有最小骨髓保留模型的部分身体照射以模拟可能保留骨髓的人类有意或意外的辐射暴露，并允许同时分析对器官系统的短期和长期损害。为了帮助了解部分身体照射模型的辐射诱导损伤的自然史，我们定量分析了非人类灵长类动物在 12 Gy 部分身体照射和 2.5% 骨髓保留和 6 MV LINAC 衍生光子后的血浆蛋白质组在 3 周的时间段内以 0.80 Gy min 为单位。通过液相色谱-串联质谱分析血浆蛋白质组。在蛋白质组数据中发现了许多趋势，包括明显的蛋白质变化以及在所有时间点和剂量水平上持续上调或下调的蛋白质变化。进行了通路和基因本体分析；生物信息学分析揭示了高剂量照射后显着的途径和生物过程扰动，并揭示了辐射损伤的潜在机制。此外，还鉴定出最有可能用作辐射照射生物标志物的蛋白质。生物信息学分析揭示了高剂量照射后显着的途径和生物过程扰动，并揭示了辐射损伤的潜在机制。此外，还鉴定出最有可能用作辐射照射生物标志物的蛋白质。生物信息学分析揭示了高剂量照射后显着的途径和生物过程扰动，并揭示了辐射损伤的潜在机制。此外，还鉴定出最有可能用作辐射照射生物标志物的蛋白质。